Isolation and characterization of an antimicrobial lipopeptide produced by Paenibacillus ehimensis MA2012.

Autor: Naing KW; Institute of Environmentally-Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Korea., Lee YS; Institute of Environmentally-Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Korea., Nguyen XH; Institute of Environmentally-Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Korea., Jeong MH; Institute of Environmentally-Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Korea., Anees M; Department of Microbiology, Kohat University of Science and Technology, Kohat, Pakistan., Oh BS; Bureau of Agriculture, Lanad and Ocean Policy, Office for Government Policy Coordination, Dasomro, Sejong, Korea., Cho JY; Department of Food Engineering, Mokpo National University, Mokpo, Korea., Moon JH; Department of Food Science and Technology and Functional Food Research Center, Chonnam National University, Gwangju, Korea., Kim KY; Institute of Environmentally-Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Korea.
Jazyk: angličtina
Zdroj: Journal of basic microbiology [J Basic Microbiol] 2015 Jul; Vol. 55 (7), pp. 857-68. Date of Electronic Publication: 2015 Jan 15.
DOI: 10.1002/jobm.201400505
Abstrakt: In this study, a novel lipopeptide antibiotic was isolated from the culture supernatant of Paenibacillus ehimensis strain MA2012. After analyses by mass spectrometry (MS), nuclear magnetic resonance (NMR), and high resolution mass spectrometry (HR-MS/MS) the compound was identified to be polypeptin C consisting of 3-hydroxy-4-methyl-hexanoic acid moiety and nine amino acids as peptide body. It has the same molecular mass (1115 Da) with that of polypeptin A and B but the amino acid positions differ. A relatively low concentration (125 ppm) of polypeptin C lowered the surface tension of water from 72.2 to 36.4 mN/m. It showed antimicrobial activity against several plant pathogenic bacteria and fungi. When the polypeptin C was applied to the ripe pepper fruits previously inoculated with conidia of Colletotrichum gloeosporioides, the hyphal growth on the fruit was significantly suppressed. Moreover, the hyphal morphology of C. gloeosporioides was greatly affected by the purified compound. All these data suggest the great potential of P. ehimensis MA2012 to control plant fungal and bacterial diseases.
(© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
Databáze: MEDLINE
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