Simultaneous determination of levodopa, carbidopa, entacapone, tolcapone, 3-O-methyldopa and dopamine in human plasma by an HPLC-MS/MS method.

Autor:	Ribeiro RP; Universidade Federal do Paraná, Department of Pharmacy, 632 Lothário Meissner Avenue, 80210-170, Curitiba - PR, Brazil., Gasparetto JC, de Oliveira Vilhena R, Guimarães de Francisco TM, Martins CA, Cardoso MA, Pontarolo R, de Carvalho KA
Jazyk:	angličtina
Zdroj:	Bioanalysis [Bioanalysis] 2015; Vol. 7 (2), pp. 207-20.
DOI:	10.4155/bio.14.230
Abstrakt:	Background: In this study, we developed and validated a HPLC-MS/MS method capable of simultaneously determining levodopa, carbidopa, entacapone, tolcapone, 3-O-methyldopa and dopamine in human plasma. RESULTS & METHODOLOGY: Chromatographic separation was achieved using a C8 column with a mobile phase consisting of a gradient of water and acetonitrile:methanol (90:10 v/v), both containing 0.1% formic acid. The developed method was selective, sensitive (LD<7.0 ng ml(-1)), linear (r>0.99), precise (RSD<11.3%), accurate (RE<11.8%) and free of residual and matrix effects. The developed method was successfully applied in plasma patients with Parkinson's disease using Stalevo®. Conclusion: The new method can be used for the clinical monitoring of these substances and applied to adjustments in drug dosages.
Databáze:	MEDLINE
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