Study of Paclitaxel-Treated HeLa Cells by Differential Electrical Impedance Flow Cytometry.

Autor:	Kirkegaard J; Department of Micro- and Nanotechnology, Technical University of Denmark, Oersteds Plads 345 East, DK-2800 Kgs. Lyngby, Denmark; E-Mails: julki@nanotech.dtu.dk (J.K.); romenrt@yahoo.es (R.R.-T.); Winnie.Svendsen@nanotech.dtu.dk (W.E.S.) ; DELTA Dansk Elektronik, Lys & Akustik, Venlighedsvej 4, DK-2970 Hørsholm, Denmark., Clausen CH; Department of Micro- and Nanotechnology, Technical University of Denmark, Oersteds Plads 345 East, DK-2800 Kgs. Lyngby, Denmark; E-Mails: julki@nanotech.dtu.dk (J.K.); romenrt@yahoo.es (R.R.-T.); Winnie.Svendsen@nanotech.dtu.dk (W.E.S.)., Rodriguez-Trujillo R; Department of Micro- and Nanotechnology, Technical University of Denmark, Oersteds Plads 345 East, DK-2800 Kgs. Lyngby, Denmark; E-Mails: julki@nanotech.dtu.dk (J.K.); romenrt@yahoo.es (R.R.-T.); Winnie.Svendsen@nanotech.dtu.dk (W.E.S.) ; DELTA Dansk Elektronik, Lys & Akustik, Venlighedsvej 4, DK-2970 Hørsholm, Denmark., Svendsen WE; Department of Micro- and Nanotechnology, Technical University of Denmark, Oersteds Plads 345 East, DK-2800 Kgs. Lyngby, Denmark; E-Mails: julki@nanotech.dtu.dk (J.K.); romenrt@yahoo.es (R.R.-T.); Winnie.Svendsen@nanotech.dtu.dk (W.E.S.).
Jazyk:	angličtina
Zdroj:	Biosensors [Biosensors (Basel)] 2014 Aug 13; Vol. 4 (3), pp. 257-72. Date of Electronic Publication: 2014 Aug 13 (Print Publication: 2014).
DOI:	10.3390/bios4030257
Abstrakt:	This work describes the electrical investigation of paclitaxel-treated HeLa cells using a custom-made microfluidic biosensor for whole cell analysis in continuous flow. We apply the method of differential electrical impedance spectroscopy to treated HeLa cells in order to elucidate the changes in electrical properties compared with non-treated cells. We found that our microfluidic system was able to distinguish between treated and non-treated cells. Furthermore, we utilize a model for electrical impedance spectroscopy in order to perform a theoretical study to clarify our results. This study focuses on investigating the changes in the electrical properties of the cell membrane caused by the effect of paclitaxel. We observe good agreement between the model and the obtained results. This establishes the proof-of-concept for the application in cell drug therapy.
Databáze:	MEDLINE
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