Seroepidemiological analysis of toxoplasmosis in college students.

Autor: Rodrigues JP; Institute of Tropical Medicine of São Paulo, University of São Paulo (USP), São Paulo, São Paulo State Brazil., Frei F; Department of Biological Sciences, (UNESP), Assis, São Paulo State Brazil., Navarro IT; Department of Veterinary Medicine, Londrina State University, Londrina, Paraná State Brazil., Silva LP; Department of Biological Sciences, (UNESP), Assis, São Paulo State Brazil., Marcelino MY; Graduate Program in Biotechnology, University of São Paulo (USP)/Butantan Institute/Institute for Technological Research (IPT), São Paulo, São Paulo State Brazil., de Andrade-Junior HF; Institute of Tropical Medicine of São Paulo, University of São Paulo (USP), São Paulo, São Paulo State Brazil., de Faria CA; Department of Genetics, Ribeirão Preto Medical School, University of São Paulo (USP), Ribeirão Preto, São Paulo State Brazil., Santos M; Department of Biological Sciences, (UNESP), Assis, São Paulo State Brazil., Ribeiro-Paes JT; Department of Biological Sciences, (UNESP), Assis, São Paulo State Brazil ; Laboratório de Genética e Terapia Celular (GenTe CeL), Departamento de Ciências Biológicas, Universidade Estadual Paulista (UNESP), Campus de Assis, Av. Dom Antônio, 2100, Assis, SP CEP 19.806-900 Brazil.
Jazyk: angličtina
Zdroj: The journal of venomous animals and toxins including tropical diseases [J Venom Anim Toxins Incl Trop Dis] 2015 Jan 05; Vol. 21 (1), pp. 1. Date of Electronic Publication: 2015 Jan 05 (Print Publication: 2015).
DOI: 10.1186/1678-9199-21-1
Abstrakt: Background: Toxoplasmosis is a zoonosis caused by an obligate intracellular parasite, Toxoplasma gondii, which affects warm-blooded animals including humans. Its prevalence rates usually vary in different regions of the planet.
Methods: In this study, an analysis of the seroprevalence of toxoplasmosis among Brazilian students was proposed by means of IgG specific antibodies detection. The presence of anti-Toxoplasma gondii antibodies by indirect fluorescent antibody test (IFAT) was also evaluated in order to compare it with enzyme-linked immunosorbent assay (ELISA) and to assess the use of 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) and o-phenylenediamine dihydrochloride chromogens.
Results: The IFAT method showed a seroprevalence of 22.3%. These results were similar to those obtained by ELISA (24.1%). The seroprevalence was directly estimated from the IgG avidity, which showed that in a sample of 112 students, three of them had acute infection, an incidence of 1.6% in the studied population.
Conclusion: In this study, the use of different chromogenic substrates in immunoenzymatic ELISA assays did not display different sensitivity in the detection of T. gondii-reagent serum. The extrapolation of results to this population must be carefully considered, since the investigation was conducted on a reduced sample. However, it allows us to emphasize the importance of careful and well prepared studies to identify risk factors for toxoplasmosis, to adopt preventive measures and to offer guidance to at-risk populations about the disease.
Databáze: MEDLINE
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