First isolation of enteroaggregative Escherichia coli O104:H4 from a diarrhea case in Argentina.

Autor:	Carbonari CC; Servicio Fisiopatogenia, Departamento de Bacteriología, Instituto Nacional de Enfermedades Infecciosas - ANLIS 'Dr. Carlos G. Malbrán', Buenos Aires, Argentina. Electronic address: ccarbonari@anlis.gov.ar., Deza N; Servicio Fisiopatogenia, Departamento de Bacteriología, Instituto Nacional de Enfermedades Infecciosas - ANLIS 'Dr. Carlos G. Malbrán', Buenos Aires, Argentina., Flores M; Laboratorio de la Dirección de Patologías Prevalentes y Epidemiología, Ministerio de Salud de la Provincia de Chubut, Rawson, Provincia de Chubut, Argentina., Gasparini A; Centro Periférico de Salud Ramón Carrillo, Hospital Zonal de Trelew, Trelew, Provincia de Chubut, Argentina., Manfredi E; Servicio Fisiopatogenia, Departamento de Bacteriología, Instituto Nacional de Enfermedades Infecciosas - ANLIS 'Dr. Carlos G. Malbrán', Buenos Aires, Argentina., Rivas M; Servicio Fisiopatogenia, Departamento de Bacteriología, Instituto Nacional de Enfermedades Infecciosas - ANLIS 'Dr. Carlos G. Malbrán', Buenos Aires, Argentina.
Jazyk:	angličtina
Zdroj:	Revista Argentina de microbiologia [Rev Argent Microbiol] 2014 Oct-Dec; Vol. 46 (4), pp. 302-6.
DOI:	10.1016/S0325-7541(14)70086-0
Abstrakt:	We describe the first isolation of an enteroaggregative Escherichia coli (EAEC) O104:H4 strain associated with an acute diarrhea case in Argentina. Two multiplex PCRs (mPCR) were performed as screening of genes mPCR1 (eae, lt, and st) and mPCR2 (IpaH, aggR, stx1 and stx2). A mPCR to detect the rfbO104, fliCH4 and terD genes, and PCR assays for the detection of pCVD432 plasmid, aaiC and lpfO113 genes were included. Biochemical and antimicrobial susceptibility assays as well as serotyping were performed. The identified E. coli strain was susceptible to all antimicrobials tested and harbored the aggR, aaiC, pCVD432 plasmid, lpfO113, rfbO104, fliCH4 and terD genes. Although serotype EAEC O104:H4 rarely spreads and sporadic cases have been reported, global concern increased after the large-scale outbreak in Europe in 2011. The finding of EAEC O104:H4 reinforces the need for improved methodologies for the detection of all E. coli pathotypes. (Copyright © 2014 Asociación Argentina de Microbiología. Publicado por Elsevier España. All rights reserved.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu