Low concentrations of cylindrospermopsin induce increases of reactive oxygen species levels, metabolism and proliferation in human hepatoma cells (HepG2).

Autor: Liebel S; Departamento de Biologia Celular, Universidade Federal do Paraná, Cx. Postal 19031, CEP 81.531-990, Curitiba, PR, Brazil. Electronic address: samliebel@yahoo.com.br., de Oliveira Ribeiro CA; Departamento de Biologia Celular, Universidade Federal do Paraná, Cx. Postal 19031, CEP 81.531-990, Curitiba, PR, Brazil., de Magalhães VF; Instituto de Biofísica Carlos Chagas Filho, Centro de Ciências da Saúde, Bloco G, Ilha do Fundão, Universidade Federal do Rio de Janeiro, CEP 21949-900, Rio de Janeiro, RJ, Brazil., da Silva Rde C; Instituto de Biofísica Carlos Chagas Filho, Centro de Ciências da Saúde, Bloco G, Ilha do Fundão, Universidade Federal do Rio de Janeiro, CEP 21949-900, Rio de Janeiro, RJ, Brazil., Rossi SC; Departamento de Farmacologia, Universidade Federal do Paraná, Cx. Postal 19031, CEP 81.531-990, Curitiba, PR, Brazil., Randi MA; Departamento de Biologia Celular, Universidade Federal do Paraná, Cx. Postal 19031, CEP 81.531-990, Curitiba, PR, Brazil., Filipak Neto F; Departamento de Biologia Celular, Universidade Federal do Paraná, Cx. Postal 19031, CEP 81.531-990, Curitiba, PR, Brazil. Electronic address: filipak@ufpr.br.
Jazyk: angličtina
Zdroj: Toxicology in vitro : an international journal published in association with BIBRA [Toxicol In Vitro] 2015 Apr; Vol. 29 (3), pp. 479-88. Date of Electronic Publication: 2015 Jan 06.
DOI: 10.1016/j.tiv.2014.12.022
Abstrakt: Human hepatoma cells (HepG2) were exposed to purified cylindrospermopsin (CYN), a potent toxicant for eukaryotic cells produced by several cyanobacteria. CYN was not toxic at concentrations up to 10 μgl(-1), leading to increased viability and metabolism in cells cultured with 10% fetal bovine serum (FBS). Reduction of FBS concentration to 2% and induction of cytochrome P450 (CYP) isoforms were performed in order to make xenobiotic-metabolizing capacity of HepG2 cells closest to that of 'normal' cells. HepG2 cells proliferated less after CYPs-induction, and this induction has lead to similar results of non-induced cells, except for few individual parameters such lipid peroxidation. Foremost, low concentrations of CYN (below or equal 10 μgl(-1)) have induced HepG2 cells proliferation and metabolism increase, which was not expected.
(Copyright © 2015 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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