Effects of transcutaneous electrical nerve stimulation on fetal and placental development in an experimental model of placental insufficiency.

Autor:	Guimarães CS; a General Pathology Discipline, Biological and Natural Sciences Institute, Triângulo Mineiro Federal University , Uberaba , Brazil ., Gomes BB; a General Pathology Discipline, Biological and Natural Sciences Institute, Triângulo Mineiro Federal University , Uberaba , Brazil ., Oliveira RA; a General Pathology Discipline, Biological and Natural Sciences Institute, Triângulo Mineiro Federal University , Uberaba , Brazil ., Yamamoto LR; a General Pathology Discipline, Biological and Natural Sciences Institute, Triângulo Mineiro Federal University , Uberaba , Brazil ., Rocha LP; a General Pathology Discipline, Biological and Natural Sciences Institute, Triângulo Mineiro Federal University , Uberaba , Brazil ., Glória MA; b Nephrology Division , Federal University of São Paulo (UNIFESP) , São Paulo , Brazil , and., Machado JR; a General Pathology Discipline, Biological and Natural Sciences Institute, Triângulo Mineiro Federal University , Uberaba , Brazil ., Câmara NO; b Nephrology Division , Federal University of São Paulo (UNIFESP) , São Paulo , Brazil , and.; c Department of Immunology , Institute of Biomedical Sciences IV, University of São Paulo (USP) , São Paulo , Brazil., Reis MA; a General Pathology Discipline, Biological and Natural Sciences Institute, Triângulo Mineiro Federal University , Uberaba , Brazil ., Corrêa RR; a General Pathology Discipline, Biological and Natural Sciences Institute, Triângulo Mineiro Federal University , Uberaba , Brazil .
Jazyk:	angličtina
Zdroj:	The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians [J Matern Fetal Neonatal Med] 2016; Vol. 29 (2), pp. 283-9. Date of Electronic Publication: 2015 Jan 08.
DOI:	10.3109/14767058.2014.999034
Abstrakt:	Objective: To elucidate the effects of transcutaneous electrical nerve stimulation (TENS) in pregnancies with placental insufficiency. Methods: Pregnant rats were subjected to uterine artery ligation and to TENS according to the following groups: ligated stimulated (LS); ligated non-stimulated (LN), control stimulated (CS); and control non-stimulated (CN). Fetal external measurements, such as crown-rump length (CRL), fronto-occipital distance (FOD), thoracic ventral-dorsal (TVDD) and abdominal ventral-dorsal (AVDD) distances were analyzed together with the area occupied by fetal internal organs. Glucose transporter 1 (GLUT-1) expression was evaluated by immunohistochemistry in fetal organs. Thickness of junctional, labyrinth and intermediate placental zones was analyzed by morphometric evaluation in HE-stained slides, and placental hypoxia-inducible factor 1 alfa expression was measured by real-time polymerase chain reaction. Results: In LN and CS groups compared to the CN group, CRL was reduced (27.51/28.95 versus 30.16 mm), as well as FOD (6.63/6.63 versus 7.36 mm), AVDD (7.38/8.00 versus 8.61 mm) and TVDD (6.46/6.87 versus 7.23 mm). Brain GLUT-1 expression was higher in LS (1.3%) and CS (1.8%). The area occupied by placental vessels in the labyrinth zone (29.67 ± 3.51 versus 20.83 ± 7.63) and intermediate zone (26.46 ± 10.21 versus 10.86 ± 8.94) was larger in the LS group than in the LN group. Conclusions: Our results suggest a negative effect of TENS on placental development, thus compromising the maintenance of adequate blood flow to the fetus.
Databáze:	MEDLINE
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