Human gut Bacteroidetes can utilize yeast mannan through a selfish mechanism.

Autor: Cuskin F; Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne NE2 4HH, U.K.; Complex Carbohydrate Research Center, The University of Georgia, 315 Riverbend Road, Athens, GA 30602, USA., Lowe EC; Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne NE2 4HH, U.K., Temple MJ; Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne NE2 4HH, U.K., Zhu Y; Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne NE2 4HH, U.K.; Complex Carbohydrate Research Center, The University of Georgia, 315 Riverbend Road, Athens, GA 30602, USA., Cameron E; Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI, USA., Pudlo NA; Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI, USA., Porter NT; Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI, USA., Urs K; Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI, USA., Thompson AJ; Department of Chemistry, University of York, York YO10 5DD, U.K., Cartmell A; School of Chemistry and Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Parkville, Victoria 3010, Australia., Rogowski A; Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne NE2 4HH, U.K., Hamilton BS; Dept. of Pharmaceutical Chemistry, University of Kansas School of Pharmacy, 2095 Constant Ave, Lawrence, KS 66047, USA., Chen R; Dept. of Pharmaceutical Chemistry, University of Kansas School of Pharmacy, 2095 Constant Ave, Lawrence, KS 66047, USA., Tolbert TJ; Dept. of Pharmaceutical Chemistry, University of Kansas School of Pharmacy, 2095 Constant Ave, Lawrence, KS 66047, USA., Piens K; Oxyrane, Ghent, Belgium., Bracke D; Oxyrane, Ghent, Belgium., Vervecken W; Oxyrane, Ghent, Belgium., Hakki Z; School of Chemistry and Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Parkville, Victoria 3010, Australia., Speciale G; School of Chemistry and Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Parkville, Victoria 3010, Australia., Munōz-Munōz JL; Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne NE2 4HH, U.K., Day A; Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne NE2 4HH, U.K., Peña MJ; Complex Carbohydrate Research Center, The University of Georgia, 315 Riverbend Road, Athens, GA 30602, USA., McLean R; Agriculture and Agri-Food Canada, Lethbridge Research Centre, Lethbridge, AB, Canada., Suits MD; Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada., Boraston AB; Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada., Atherly T; USDA, Agricultural Research Service, National Laboratory for Agriculture and the Environment, Ames, Iowa, USA., Ziemer CJ; USDA, Agricultural Research Service, National Laboratory for Agriculture and the Environment, Ames, Iowa, USA., Williams SJ; School of Chemistry and Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Parkville, Victoria 3010, Australia., Davies GJ; Department of Chemistry, University of York, York YO10 5DD, U.K., Abbott DW; Complex Carbohydrate Research Center, The University of Georgia, 315 Riverbend Road, Athens, GA 30602, USA.; Agriculture and Agri-Food Canada, Lethbridge Research Centre, Lethbridge, AB, Canada., Martens EC; Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI, USA., Gilbert HJ; Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne NE2 4HH, U.K.; Complex Carbohydrate Research Center, The University of Georgia, 315 Riverbend Road, Athens, GA 30602, USA.
Jazyk: angličtina
Zdroj: Nature [Nature] 2015 Jan 08; Vol. 517 (7533), pp. 165-169.
DOI: 10.1038/nature13995
Abstrakt: Yeasts, which have been a component of the human diet for at least 7,000 years, possess an elaborate cell wall α-mannan. The influence of yeast mannan on the ecology of the human microbiota is unknown. Here we show that yeast α-mannan is a viable food source for the Gram-negative bacterium Bacteroides thetaiotaomicron, a dominant member of the microbiota. Detailed biochemical analysis and targeted gene disruption studies support a model whereby limited cleavage of α-mannan on the surface generates large oligosaccharides that are subsequently depolymerized to mannose by the action of periplasmic enzymes. Co-culturing studies showed that metabolism of yeast mannan by B. thetaiotaomicron presents a 'selfish' model for the catabolism of this difficult to breakdown polysaccharide. Genomic comparison with B. thetaiotaomicron in conjunction with cell culture studies show that a cohort of highly successful members of the microbiota has evolved to consume sterically-restricted yeast glycans, an adaptation that may reflect the incorporation of eukaryotic microorganisms into the human diet.
Databáze: MEDLINE