Microwave exposure impairs synaptic plasticity in the rat hippocampus and PC12 cells through over-activation of the NMDA receptor signaling pathway.

Autor:	Xiong L; Beijing Institute of Radiation Medicine, Beijing 100850, China., Sun CF; Beijing Institute of Radiation Medicine, Beijing 100850, China., Zhang J; Beijing Institute of Radiation Medicine, Beijing 100850, China., Gao YB; Beijing Institute of Radiation Medicine, Beijing 100850, China., Wang LF; Beijing Institute of Radiation Medicine, Beijing 100850, China., Zuo HY; Beijing Institute of Radiation Medicine, Beijing 100850, China., Wang SM; Beijing Institute of Radiation Medicine, Beijing 100850, China., Zhou HM; Beijing Institute of Radiation Medicine, Beijing 100850, China., Xu XP; Beijing Institute of Radiation Medicine, Beijing 100850, China., Dong J; Beijing Institute of Radiation Medicine, Beijing 100850, China., Yao BW; Beijing Institute of Radiation Medicine, Beijing 100850, China., Zhao L; Beijing Institute of Radiation Medicine, Beijing 100850, China., Peng RY; Beijing Institute of Radiation Medicine, Beijing 100850, China.
Jazyk:	angličtina
Zdroj:	Biomedical and environmental sciences : BES [Biomed Environ Sci] 2015 Jan; Vol. 28 (1), pp. 13-24.
DOI:	10.3967/bes2015.002
Abstrakt:	Objective: The aim of this study is to investigate whether microwave exposure would affect the N-methyl-D-aspartate receptor (NMDAR) signaling pathway to establish whether this plays a role in synaptic plasticity impairment. Methods: 48 male Wistar rats were exposed to 30 mW/cm2 microwave for 10 min every other day for three times. Hippocampal structure was observed through H&E staining and transmission electron microscope. PC12 cells were exposed to 30 mW/cm2 microwave for 5 min and the synapse morphology was visualized with scanning electron microscope and atomic force microscope. The release of amino acid neurotransmitters and calcium influx were detected. The expressions of several key NMDAR signaling molecules were evaluated. Results: Microwave exposure caused injury in rat hippocampal structure and PC12 cells, especially the structure and quantity of synapses. The ratio of glutamic acid and gamma-aminobutyric acid neurotransmitters was increased and the intracellular calcium level was elevated in PC12 cells. A significant change in NMDAR subunits (NR1, NR2A, and NR2B) and related signaling molecules (Ca2+/calmodulin-dependent kinase II gamma and phosphorylated cAMP-response element binding protein) were examined. Conclusion: 30 mW/cm2 microwave exposure resulted in alterations of synaptic structure, amino acid neurotransmitter release and calcium influx. NMDAR signaling molecules were closely associated with impaired synaptic plasticity. (Copyright © 2015 The Editorial Board of Biomedical and Environmental Sciences. Published by China CDC. All rights reserved.)
Databáze:	MEDLINE
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