3,3'-Diamino-N-methyldipropylamine as a versatile affinity ligand.
| Autor: | Silva MS; CICS-UBI-Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior, Covilhã, Portugal., Graça VC, Reis LV, Santos PF, Silvestre S, Granadeiro L, Almeida P, Queiroz JA, Sousa F |
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| Jazyk: | angličtina |
| Zdroj: | Journal of separation science [J Sep Sci] 2015 Mar; Vol. 38 (5), pp. 732-40. Date of Electronic Publication: 2015 Feb 03. |
| DOI: | 10.1002/jssc.201400656 |
| Abstrakt: | Currently, in biomedicine and biotechnology fields, there is a growing need to develop and produce biomolecules with a high degree of purity. To accomplish this goal, new purification methods are being developed looking for higher performance, efficiency, selectivity, and cost-effectiveness. Affinity chromatography is considered one of the most highly selective methods for biomolecules purification. The purpose of this work is to explore a new type of a structurally simple ligand immobilized onto an agarose matrix to be used in affinity chromatography. The ligand in this study, 3,3'-diamino-N-methyldipropylamine has shown low toxicity and low cost of preparation. Moreover, the ability of the ligand to be used in affinity chromatography to purify proteins and nucleic acids was verified. An increasing sodium chloride gradient, using salt concentrations up to 500 mM, was suitable to accomplish the purification of these biomolecules, meaning that the new support allows the recovery of target biomolecules under mild conditions. Thus, the 3,3'-diamino-N-methyldipropylamine ligand is shown to be a useful and versatile tool in chromatographic experiments, with very good results either for proteins or supercoiled plasmid isoform purification. (© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.) |
| Databáze: | MEDLINE |
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