Fluorescent in situ hybridization of pre-incubated blood culture material for the rapid diagnosis of histoplasmosis.

Autor:	da Silva RM Jr; Universidade do Estado do Amazonas, Manaus, Brazil., da Silva Neto JR; Fundação de Medicina Tropical Dr. Heitor Vieira Dourado, Manaus, Brazil., Santos CS; Fundação de Medicina Tropical Dr. Heitor Vieira Dourado, Manaus, Brazil., Cruz KS; Fundação de Medicina Tropical Dr. Heitor Vieira Dourado, Manaus, Brazil., Frickmann H; Department of Tropical Medicine at the Bernhard Nocht Institute, German Armed Forces Hospital of Hamburg, Hamburg, Germany., Poppert S; Institute of Medical Microbiology, Justus-Liebig-University Giessen, Giessen, Germany., Koshikene D; Instituto Nacional de Pesquisas da Amazônia, Manaus, Brazil., de Souza JV; Instituto Nacional de Pesquisas da Amazônia, Manaus, Brazil joao.souza@inpa.gov.br.
Jazyk:	angličtina
Zdroj:	Medical mycology [Med Mycol] 2015 Feb 01; Vol. 53 (2), pp. 160-4. Date of Electronic Publication: 2014 Dec 23.
DOI:	10.1093/mmy/myu080
Abstrakt:	Fluorescence in situ hybridization (FISH) has been shown to be useful for the detection of Candida and Cryptococcus species in blood culture materials. FISH procedures for the detection of Histoplasma capsulatum var. capsulatum have not been reported so far. This study describes the development and evaluation of fluorescently labeled rRNA-targeting FISH probes to detect and identify H. capsulatum in blood cultures. All three analyzed H. capsulatum reference strains and clinical isolates showed positive signals with the newly designed specific oligonucleotide probes for H. capsulatum, whereas negative reactions were observed for all three nontarget yeast species and the two nontarget bacteria. The assay was also successfully applied for detections of H. capsulatum cells in pre-incubated blood culture samples of patients with clinical suspicion of histoplasmosis (n = 33). The described FISH-based assay was shown to be easy to apply, sensitive, and specific (compared to polymerase chain reaction) for the detection and identification of H. capsulatum in this proof-of-principle analysis. Larger multicentric assessments are recommended for a thorough diagnostic evaluation of the procedure. (© The Author 2014. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
Databáze:	MEDLINE
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