Investigation of modification X-ray induced bystander effect in vitro.

Autor:	Shemetun OV; State Institution National Research Center for Radiation Medicine of the National Academy of Medical Sciences of Ukraine, Melnykov str., 53, Kyiv, 04050, Ukraine. vkpand@ukr.net., Talan OO; State Institution National Research Center for Radiation Medicine of the National Academy of Medical Sciences of Ukraine, Melnykov str., 53, Kyiv, 04050, Ukraine. vkpand@ukr.net.
Jazyk:	English; Ukrainian
Zdroj:	Problemy radiatsiinoi medytsyny ta radiobiolohii [Probl Radiac Med Radiobiol] 2014 Sep; Vol. 19, pp. 371-6.
Abstrakt:	Unlabelled: Objective - to investigate the modification of bystander effect induced by X-irradiation of human peripheral blood in vitro by application of antioxidant vitamin medication. Material and methods. Modeling of radiation-induced bystander effect in vitro in mixed lymphocyte cultures exposed to dose of 1 Gy and non-irradiated blood lymphocytes of persons of different sexes, GTG-staining of metaphase chromosomes and their cytogenetic analysis; application of antioxidant preparation (soluble forms of vitamins E, C and A) in concentration 40 μg/ml. Results. Under the introduction of antioxidant preparation into mixed culture before lymphocytes cultivation frequency of chromosomal aberrations in bystander cells did not significantly different from the control (p > 0.05). Conclusions: application of antioxidant preparation modifies the radiation-induced bystander effect in unirradiated human peripheral blood lymphocytes under their joint cultivation with lymphocytes irradiated in dose of 1 Gy. Antioxidant prevents the development of secondary oxidative stress in unirradiated cells, eliminates the development in them of radiation-induced bystander effect and ensures the preservation of stability of their chromosome apparatus. (O. V. Shemetun, O. O. Talan.)
Databáze:	MEDLINE
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