| Autor: |
Muschietti JP; Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Buenos Aires, Argentina., Bianchini GM, Martinetto HE, Carricarte VC, Giusto N, Farber DB, Torres HN, Flawia MM |
| Jazyk: |
angličtina |
| Zdroj: |
European journal of biochemistry [Eur J Biochem] 1989 Oct 20; Vol. 185 (1), pp. 205-10. |
| DOI: |
10.1111/j.1432-1033.1989.tb15103.x |
| Abstrakt: |
Adenylate cyclase catalytic subunits from Neurospora crassa membranes may interact with regulatory factors from membranes of bovine retinal rod outer segments (pretreated with N-ethylmaleimide), reconstituting a heterologous system which, in the presence of light, is catalytically active in assay mixtures containing MgATP. Maximal activation was observed at 550 nm. Transducin-depleted retinal membranes were not capable of reconstituting the heterologous light-stimulated adenylate cyclase system. Addition of a transducin preparation to depleted membranes restored the reconstitution capacity of these membranes. A similar heterologous adenylate cyclase system was reconstituted with Neurospora and mouse retinal whole membranes (pretreated with N-ethylmaleimide). Membranes from mice suffering photoreceptor degeneration (rd homozygotes) did not reconstitute an heterologous adenylate cyclase system. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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