Rapid and accurate detection of urinary pathogens by mobile IMS-based electronic nose: a proof-of-principle study.

Autor:	Roine A; School of Medicine, University of Tampere, Tampere, Finland., Saviauk T; School of Medicine, University of Tampere, Tampere, Finland., Kumpulainen P; Department of Automation Science and Engineering, Tampere University of Technology, Tampere, Finland., Karjalainen M; Department of Automation Science and Engineering, Tampere University of Technology, Tampere, Finland., Tuokko A; School of Medicine, University of Tampere, Tampere, Finland., Aittoniemi J; Department of Clinical Microbiology, Fimlab Laboratories, Tampere, Finland., Vuento R; Department of Clinical Microbiology, Fimlab Laboratories, Tampere, Finland., Lekkala J; Department of Automation Science and Engineering, Tampere University of Technology, Tampere, Finland., Lehtimäki T; Department of Clinical Chemistry, Fimlab Laboratories and University of Tampere, School of Medicine, Tampere, Finland., Tammela TL; Department of Surgery, School of Medicine, University of Tampere and Department of Urology, Tampere University Hospital, Tampere, Finland., Oksala NK; Department of Surgery, School of Medicine, University of Tampere and Department of Vascular Surgery, Tampere University Hospital, Tampere, Finland.
Jazyk:	angličtina
Zdroj:	PloS one [PLoS One] 2014 Dec 19; Vol. 9 (12), pp. e114279. Date of Electronic Publication: 2014 Dec 19 (Print Publication: 2014).
DOI:	10.1371/journal.pone.0114279
Abstrakt:	Unlabelled: Urinary tract infection (UTI) is a common disease with significant morbidity and economic burden, accounting for a significant part of the workload in clinical microbiology laboratories. Current clinical chemisty point-of-care diagnostics rely on imperfect dipstick analysis which only provides indirect and insensitive evidence of urinary bacterial pathogens. An electronic nose (eNose) is a handheld device mimicking mammalian olfaction that potentially offers affordable and rapid analysis of samples without preparation at athmospheric pressure. In this study we demonstrate the applicability of ion mobility spectrometry (IMS) -based eNose to discriminate the most common UTI pathogens from gaseous headspace of culture plates rapidly and without sample preparation. We gathered a total of 101 culture samples containing four most common UTI bacteries: E. coli, S. saprophyticus, E. faecalis, Klebsiella spp and sterile culture plates. The samples were analyzed using ChemPro 100i device, consisting of IMS cell and six semiconductor sensors. Data analysis was conducted by linear discriminant analysis (LDA) and logistic regression (LR). The results were validated by leave-one-out and 5-fold cross validation analysis. In discrimination of sterile and bacterial samples sensitivity of 95% and specificity of 97% were achieved. The bacterial species were identified with sensitivity of 95% and specificity of 96% using eNose as compared to urine bacterial cultures. In Conclusion: These findings strongly demonstrate the ability of our eNose to discriminate bacterial cultures and provides a proof of principle to use this method in urinanalysis of UTI.
Databáze:	MEDLINE
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