| Autor: |
Zakhidov ST, Pavliuchenkova SM, Samoĭlov AV, Mudzhiri NM, Marshak TL, Rudoĭ VM, Dement'eva OV, Zelenina IA, Skuridin SG, Evdokimov IuM |
| Jazyk: |
ruština |
| Zdroj: |
Izvestiia Akademii nauk. Seriia biologicheskaia [Izv Akad Nauk Ser Biol] 2013 Nov-Dec (6), pp. 645-52. |
| Abstrakt: |
The response of ejaculated bovine spermatozoa to gold nanoparticles was studied by the standard method of nuclear chromatin decondensation in vitro. After the treatment of semen samples with a hydrosol containing gold nanoparticles with an average diameter of 3.0 nm and a concentration of 1 x 10(15) particles/mL, the ability of sperm nuclei to decondense in the presence of sodium dodecyl sulfate (SDS) and dithiothreitol (DTT) dramatically changed compared to the control. The frequencies of gametes with nondecondensed ("intact"), partially decondensed, and completely decondensed nuclei correlated as 40 : 32 : 28% and 0 : 36 : 64% in the experiment and the control, respectively. Moreover, the appearance of a sufficiently large number of gametes with destructed and almost completely destroyed nuclei was noticed in the spermatozoa treated with gold nanoparticles. This article suggests the putative mechanisms of action of ultrasmall gold nanoparticles on the structural and functional integrity of the deoxyribonucleoprotein (DNP) complex of mature male gametes. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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