Molecular characteristics of rotavirus isolated from a diarrhea outbreak in october 2008 in bintuni bay, papua, indonesia.

Autor: Pratiwi E; Center for Biomedical and Basic Technology of Health, National Institute of Health Research and Development, Ministry of Health, Jakarta, Indonesia., Setiawaty V; Center for Biomedical and Basic Technology of Health, National Institute of Health Research and Development, Ministry of Health, Jakarta, Indonesia., Putranto RH; Center for Biomedical and Basic Technology of Health, National Institute of Health Research and Development, Ministry of Health, Jakarta, Indonesia.
Jazyk: angličtina
Zdroj: Virology : research and treatment [Virology (Auckl)] 2014 Mar 09; Vol. 5, pp. 11-4. Date of Electronic Publication: 2014 Mar 09 (Print Publication: 2014).
DOI: 10.4137/VRT.S13555
Abstrakt: Background: Viral diarrhea continues to be a health problem in Indonesia that often causes outbreaks; in particular, acute viral diarrhea in young children. Rotavirus is the leading cause of severe diarrhea in children under two years of age. This study aimed to determine the genotypes of rotavirus in Bintuni Bay, Papua.
Methods: Stool specimens from 15 patients were collected and analyzed for rotavirus using an enzyme immunosorbent assay (EIA) and reverse transcriptase-polymerase chain reaction (RT-PCR). Subsequently, we sequenced the genetic material of rotavirus positive samples by RT-PCR and analyzed the results using Mega-4 software.
Results: Two rotavirus serotypes were identified from the diarrhea outbreak in Bintuni, Papua in October 2008: serotype G1 with G1P[6] (50%) and G1P[8] (16.7%) strains, and serotype G2 with G2P[4] (23.3%) strain. Phylogenetic tree analyses of VP7 protein showed that rotavirus-infected diarrhea in Bintuni Bay, Papua at that time was dominated by the G1 serotype (83%).
Conclusion: The laboratory results showed that G1 serotype rotavirus was a cause of the outbreak of diarrhea in October 2008 in Bintuni, Papua.
Databáze: MEDLINE
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