Effect of Uncaria tomentosa Extract on Apoptosis Triggered by Oxaliplatin Exposure on HT29 Cells.

Autor: de Oliveira LZ; University Hospital of Santa Maria, Federal University of Santa Maria (UFSM), Avenida Roraima, Prédio 20, 97105-900 Santa Maria, RS, Brazil ; Postgraduate Program in Pharmaceutical Sciences, UFSM, 97105-900 Santa Maria, RS, Brazil., Farias IL; University Hospital of Santa Maria, Federal University of Santa Maria (UFSM), Avenida Roraima, Prédio 20, 97105-900 Santa Maria, RS, Brazil., Rigo ML; Laboratory of Biotechnology and Animal Reproduction, UFSM, 97105-900 Santa Maria, RS, Brazil., Glanzner WG; Laboratory of Biotechnology and Animal Reproduction, UFSM, 97105-900 Santa Maria, RS, Brazil., Gonçalves PB; Laboratory of Biotechnology and Animal Reproduction, UFSM, 97105-900 Santa Maria, RS, Brazil., Cadoná FC; Postgraduate Program in Biochemistry and Toxicology, UFSM, 97105-900 Santa Maria, RS, Brazil., Cruz IB; Postgraduate Program in Biochemistry and Toxicology, UFSM, 97105-900 Santa Maria, RS, Brazil., Farias JG; Postgraduate Program in Agronomy, UFSM, 97105-900 Santa Maria, RS, Brazil., Duarte MM; Lutheran University of Brazil (ULBRA), BR 287, Km 252, 97020-001 Santa Maria, RS, Brazil., Franco L; Herbarium Laboratório Botânico Ltda., Avenida Santos Dumont 1100, 83403-500 Colombo, PR, Brazil., Bertol G; Herbarium Laboratório Botânico Ltda., Avenida Santos Dumont 1100, 83403-500 Colombo, PR, Brazil., Colpo E; Postgraduate Program in Biochemistry and Toxicology, UFSM, 97105-900 Santa Maria, RS, Brazil., Brites PC; University Hospital of Santa Maria, Federal University of Santa Maria (UFSM), Avenida Roraima, Prédio 20, 97105-900 Santa Maria, RS, Brazil., Rocha JB; Postgraduate Program in Biochemistry and Toxicology, UFSM, 97105-900 Santa Maria, RS, Brazil., Leal DB; Postgraduate Program in Pharmaceutical Sciences, UFSM, 97105-900 Santa Maria, RS, Brazil.
Jazyk: angličtina
Zdroj: Evidence-based complementary and alternative medicine : eCAM [Evid Based Complement Alternat Med] 2014; Vol. 2014, pp. 274786. Date of Electronic Publication: 2014 Nov 11.
DOI: 10.1155/2014/274786
Abstrakt: Background/Aim. The use of herbal products as a supplement to minimize the effects of chemotherapy for cancer treatment requires further attention with respect to the activity and toxicity of chemotherapy. Uncaria tomentosa extract, which contains oxindole alkaloids, is one of these herbal products. The objective of this study was to evaluate whether Uncaria tomentosa extract modulates apoptosis induced by chemotherapy exposure. Materials and Methods. Colorectal adenocarcinoma cells (HT29 cells) were grown in the presence of oxaliplatin and/or Uncaria tomentosa extract. Results. The hydroalcoholic extract of Uncaria tomentosa enhanced chemotherapy-induced apoptosis, with an increase in the percentage of Annexin positive cells, an increase in caspase activities, and an increase of DNA fragments in culture of the neoplastic cells. Moreover, antioxidant activity may be related to apoptosis. Conclusion. Uncaria tomentosa extract has a role for cancer patients as a complementary therapy. Further studies evaluating these beneficial effects with other chemotherapy drugs are recommended.
Databáze: MEDLINE
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