Kinesin-13 regulates the quantity and quality of tubulin inside cilia.

Autor:	Vasudevan KK; Department of Cellular Biology, University of Georgia, Athens, GA 30602;, Jiang YY; Department of Cellular Biology, University of Georgia, Athens, GA 30602;, Lechtreck KF; Department of Cellular Biology, University of Georgia, Athens, GA 30602;, Kushida Y; Department of Structural Biosciences, University of Tsukuba, Tsukuba, Ibaraki 305-8577, Japan;, Alford LM; Department of Cell Biology, Emory University, Atlanta, GA 30303;, Sale WS; Department of Cell Biology, Emory University, Atlanta, GA 30303;, Hennessey T; Department of Biological Sciences, State University of New York at Buffalo, Buffalo, NY 14260., Gaertig J; Department of Cellular Biology, University of Georgia, Athens, GA 30602; jgaertig@uga.edu.
Jazyk:	angličtina
Zdroj:	Molecular biology of the cell [Mol Biol Cell] 2015 Feb 01; Vol. 26 (3), pp. 478-94. Date of Electronic Publication: 2014 Dec 10.
DOI:	10.1091/mbc.E14-09-1354
Abstrakt:	Kinesin-13, an end depolymerizer of cytoplasmic and spindle microtubules, also affects the length of cilia. However, in different models, depletion of kinesin-13 either lengthens or shortens cilia, and therefore the exact function of kinesin-13 in cilia remains unclear. We generated null mutations of all kinesin-13 paralogues in the ciliate Tetrahymena. One of the paralogues, Kin13Ap, localizes to the nuclei and is essential for nuclear divisions. The remaining two paralogues, Kin13Bp and Kin13Cp, localize to the cell body and inside assembling cilia. Loss of both Kin13Bp and Kin13Cp resulted in slow cell multiplication and motility, overgrowth of cell body microtubules, shortening of cilia, and synthetic lethality with either paclitaxel or a deletion of MEC-17/ATAT1, the α-tubulin acetyltransferase. The mutant cilia assembled slowly and contained abnormal tubulin, characterized by altered posttranslational modifications and hypersensitivity to paclitaxel. The mutant cilia beat slowly and axonemes showed reduced velocity of microtubule sliding. Thus kinesin-13 positively regulates the axoneme length, influences the properties of ciliary tubulin, and likely indirectly, through its effects on the axonemal microtubules, affects the ciliary dynein-dependent motility. (© 2015 Vasudevan et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).)
Databáze:	MEDLINE
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