Assessment of antiproliferative activity of pectic substances obtained by different extraction methods from rapeseed cake on cancer cell lines.

Autor: Cobs-Rosas M; Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, General Cruz 34, Valparaíso, Chile., Concha-Olmos J; Escuela de Química y Farmacia, Facultad de Farmacia, Universidad de Valparaíso, Gran Bretaña 1093, Playa Ancha, Valparaíso, Chile. Electronic address: jacqueline.concha@uv.cl., Weinstein-Oppenheimer C; Escuela de Química y Farmacia, Facultad de Farmacia, Universidad de Valparaíso, Gran Bretaña 1093, Playa Ancha, Valparaíso, Chile; Centro Regional de Estudio en Alimentos Saludables, R12C1001, Av. Universidad 330, Curauma, Valparaíso, Chile., Zúñiga-Hansen ME; Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, General Cruz 34, Valparaíso, Chile; Centro Regional de Estudio en Alimentos Saludables, R12C1001, Av. Universidad 330, Curauma, Valparaíso, Chile.
Jazyk: angličtina
Zdroj: Carbohydrate polymers [Carbohydr Polym] 2015 Mar 06; Vol. 117, pp. 923-932. Date of Electronic Publication: 2014 Oct 22.
DOI: 10.1016/j.carbpol.2014.10.027
Abstrakt: In this work the antiproliferative activity of pectic substances obtained by different extraction methods from defatted rapeseed cake was assessed on cancer cell lines. The process consisted of sequential treatment with alkalized water (pH∼8), EDTA (0.01 M), alkaline protease (Alkalase 2.4L) and a commercial pectinase preparation (Viscozyme L or Pectinex Ultra SP-L). Pectic extracts identification was performed using spectroscopy and chromatography techniques. FT-IR and HPLC-IR results suggest that the neutral pectic extracts produced would be arabinogalactans and β-galactans. All the pectic substances extracted (acid and neutral) from RSC exhibited antiproliferative activity, being more effective on MCF-7 cells than Caco-2. The most effective pectic extract was obtained by Alkalase 2.4 L which killed over 80% of MCF-7 cells and 60% of Caco-2 cells. At less than 10 mg/mL pectic extracts enriched in neutral sugars also exhibited antiproliferative activity (50 and 40%, respectively), which was superior to the modified citric pectins activity at the same concentration for the breast cancer cell line (61.6% for MCF-7 and 49.9% for Caco-2 cells). These results show that the antiproliferative activity depends on both the type of pectin (acid or neutral) and the extraction procedure.
(Copyright © 2014 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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