Acetogenic mixotrophy: novel options for yield improvement in biofuels and biochemicals production.

Autor: Fast AG; Molecular Biotechnology Laboratory, Department of Chemical & Biomolecular Engineering, & the Delaware Biotechnology Institute, University of Delaware, Newark, DE 19711, USA., Schmidt ED; Molecular Biotechnology Laboratory, Department of Chemical & Biomolecular Engineering, & the Delaware Biotechnology Institute, University of Delaware, Newark, DE 19711, USA., Jones SW; Elcriton, Inc., 15 Reads Way, New Castle, DE 19720, USA., Tracy BP; Elcriton, Inc., 15 Reads Way, New Castle, DE 19720, USA. Electronic address: bryantracy@elcriton.com.
Jazyk: angličtina
Zdroj: Current opinion in biotechnology [Curr Opin Biotechnol] 2015 Jun; Vol. 33, pp. 60-72. Date of Electronic Publication: 2014 Dec 10.
DOI: 10.1016/j.copbio.2014.11.014
Abstrakt: Mass yields of biofuels and chemicals from sugar fermentations are limited by the decarboxylation reactions involved in Embden-Meyerhof-Parnas (EMP) glycolysis. This paper reviews one route to recapture evolved CO2 using the Wood-Ljungdahl carbon fixation pathway (WLP) in a process called anaerobic, non-photosynthetic (ANP) mixotrophic fermentation. In ANP mixotrophic fermentation, the two molecules of CO2 and eight electrons produced from glycolysis are used by the WLP to generate three molecules of acetyl-CoA from glucose, rather than the two molecules that are produced by typical fermentation processes. In this review, we define the bounds of ANP mixotrophy, calculate the potential metabolic advantages, and discuss the viability in a number of host organisms. Additionally, we highlight recent accomplishments in the field, including the recent discovery of electron bifurcation in acetogens, and close with recommendations to realize mixotrophic biofuel and biochemical production.
(Copyright © 2014 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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