Temperature-dependent transformation of biogas-producing microbial communities points to the increased importance of hydrogenotrophic methanogenesis under thermophilic operation.

Autor: Pap B; Seqomics Biotechnology Ltd., Vállalkozók útja 7, H-6782 Mórahalom, Hungary. Electronic address: bernadett.pap@gmail.com., Györkei Á; Institute of Biochemistry, Biological Research Center, Hungarian Academy of Sciences, Temesvári krt. 62, H-6726 Szeged, Hungary. Electronic address: gyorkei.adam@gmail.com., Boboescu IZ; Politehnica University of Timisoara, Hydrotechnical Engineering Dept., George Enescu str. 1/A, 300022 Timisoara, Romania. Electronic address: boboescu.iulian@yahoo.com., Nagy IK; Institute of Biochemistry, Biological Research Center, Hungarian Academy of Sciences, Temesvári krt. 62, H-6726 Szeged, Hungary. Electronic address: nagyildi@baygen.hu., Bíró T; Szent István University, Faculty of Economics, Agricultural and Health Studies, Szabadság út 1-3, H-5540 Szarvas, Hungary. Electronic address: biro.tibor@gk.szie.hu., Kondorosi É; Institute of Biochemistry, Biological Research Center, Hungarian Academy of Sciences, Temesvári krt. 62, H-6726 Szeged, Hungary. Electronic address: eva.kondorosi@gmail.com., Maróti G; Institute of Biochemistry, Biological Research Center, Hungarian Academy of Sciences, Temesvári krt. 62, H-6726 Szeged, Hungary; Politehnica University of Timisoara, Hydrotechnical Engineering Dept., George Enescu str. 1/A, 300022 Timisoara, Romania. Electronic address: marotig@baygen.hu.
Jazyk: angličtina
Zdroj: Bioresource technology [Bioresour Technol] 2015 Feb; Vol. 177, pp. 375-80. Date of Electronic Publication: 2014 Nov 20.
DOI: 10.1016/j.biortech.2014.11.021
Abstrakt: Stability of biogas production is highly dependent on the microbial community composition of the bioreactors. This composition is basically determined by the nature of biomass substrate and the physical-chemical parameters of the anaerobic digestion. Operational temperature is a major factor in the determination of the anaerobic degradation process. Next-generation sequencing (NGS)-based metagenomic approach was used to monitor the organization and operation of the microbial community throughout an experiment where mesophilic reactors (37°C) were gradually switched to thermophilic (55°C) operation. Temperature adaptation resulted in a clearly thermophilic community having a generally decreased complexity compared to the mesophilic system. A temporary destabilization of the system was observed, indicating a lag phase in the community development in response to temperature stress. Increased role of hydrogenotrophic methanogens under thermophilic conditions was shown, as well as considerably elevated levels of Fe-hydrogenases and hydrogen producer bacteria were observed in the thermophilic system.
(Copyright © 2014 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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