| Autor: |
de Paula DA; Universidade Federal de Mato Grosso CuiabáMT Brazil Universidade Federal de Mato Grosso, Cuiabá, MT, Brazil., Rosa e Silva LK; Centro de Biotecnologia Universidade Federal do Rio Grande do Sul Porto AlegreRS Brazil Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil., Staats CC; Centro de Biotecnologia Universidade Federal do Rio Grande do Sul Porto AlegreRS Brazil Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil., Vainstein MH; Centro de Biotecnologia Universidade Federal do Rio Grande do Sul Porto AlegreRS Brazil Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil., Joanoni AL; Universidade Federal de Mato Grosso CuiabáMT Brazil Universidade Federal de Mato Grosso, Cuiabá, MT, Brazil., Nakazato L; Universidade Federal de Mato Grosso CuiabáMT Brazil Universidade Federal de Mato Grosso, Cuiabá, MT, Brazil., Dutra V; Universidade Federal de Mato Grosso CuiabáMT Brazil Universidade Federal de Mato Grosso, Cuiabá, MT, Brazil. |
| Abstrakt: |
Cryptococcus neoformans and C. gattii are pathogenic yeasts that cause life-threatening diseases in humans and animals. Iron is an essential nutrient for virtually every organism as it functions as a cofactor in numerous essential enzymatic reactions. In the literature, the competition for iron between microbes and mammalian hosts during infection is well documented. In this study, we used representational difference analysis (RDA) in order to gain a better understanding of how C. gattii responds to iron starvation. A total of 15 and 29 genes were identified as having altered expression levels due to iron depletion after 3 h and 12 h, respectively. Of these, eight genes were identified in both libraries. The transcripts were related to many biological processes, such as cell cycle, ergosterol metabolism, cell wall organization, transportation, translation, cell respiration and the stress response. These data suggest a remodeling of C. gattii metabolism during conditions of iron deprivation. |
