| Autor: |
Arystarkhova EA; Shemyakin Institute of Bioorganic Chemistry, USSR Academy of Sciences, Moscow., Lakhtina OE, Modyanov NN |
| Jazyk: |
angličtina |
| Zdroj: |
FEBS letters [FEBS Lett] 1989 Jul 03; Vol. 250 (2), pp. 545-8. |
| DOI: |
10.1016/0014-5793(89)80793-8 |
| Abstrakt: |
At least three types of mRNA of the catalytic subunit of Na,K-ATPase namely alpha-,alpha+- and alpha 3-isoforms are identified in different tissues. Only two of them alpha and alpha+ have well known structural and catalytic properties. Here we present immunochemical data indicating that the alpha 3 protein really exists in pig and human kidney, and human brain. Crude membrane fractions and purified membrane-bound Na,K-ATPases were immunoblotted with alpha 3-specific antibodies raised against the synthetic peptide corresponding to the unique sequence of this isoform. The mature alpha 3-subunit is shown to include the sequence GDKKDDKSSPK followed by the initiating methionine residue. Nephron collecting tubules are proposed to specifically contain Na,K-ATPase alpha 3-isoform. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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