High frequency of Merkel cell polyomavirus DNA in the urine of kidney transplant recipients and healthy controls.

Autor: Signorini L; Department of Biomedical, Surgical and Dental Sciences, University of Milano, Milano, Italy., Belingheri M; Nephrology, Dialysis and Transplantation Unit, Clinica Pediatrica De Marchi, Milano, Italy., Ambrogi F; Department of Clinical Sciences and Community Health, University of Milano, Milano, Italy., Pagani E; Laboratory of Microbiology and Virology, Comprensorio Sanitario di Bolzano, Azienda Sanitaria dell'Alto Adige, Bolzano, Italy., Binda S; Department of Biomedical Sciences for Health, University of Milan, Italy., Ticozzi R; Department of Biomedical, Surgical and Dental Sciences, University of Milano, Milano, Italy., Ferraresso M; Department of Surgical Sciences, University of Milan, Milano, Italy., Ghio L; Nephrology, Dialysis and Transplantation Unit, Clinica Pediatrica De Marchi, Milano, Italy., Giacon B; Nephrology and Dialysis Unit, Comprensorio Sanitario di Bolzano, Azienda Sanitaria dell'Alto Adige, Bolzano, Italy., Ferrante P; Department of Biomedical, Surgical and Dental Sciences, University of Milano, Milano, Italy; Ettore Sansavini Foundation, Health Science Foundation, Lugo, RA, Italy., Delbue S; Department of Biomedical, Surgical and Dental Sciences, University of Milano, Milano, Italy; Istituto Clinico Città Studi, Milano, Italy. Electronic address: Serena.delbue@unimi.it.
Jazyk: angličtina
Zdroj: Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology [J Clin Virol] 2014 Dec; Vol. 61 (4), pp. 565-70. Date of Electronic Publication: 2014 Oct 24.
DOI: 10.1016/j.jcv.2014.10.012
Abstrakt: Background: Polyomavirus (PyV) infection is common, ranging from 60% to 100% depending on the virus. The urinary excretion rates of JC virus (JCV) and BK virus (BKV) have been extensively studied, but less is known about the more recently discovered PyVs.
Objectives: To investigate the urinary excretion of Merkel cell PyV (MCPyV), which is associated with Merkel cell carcinoma (MCC), in kidney transplant recipients and healthy subjects, as well as those of lymphotropic polyomavirus (LPV), which was isolated from the B-cells of African green monkeys but has also been found in human blood, JCV and BKV.
Study Design: Urine samples were collected from 62 adult (ATP) and 46 pediatric (PTP) kidney transplant recipients and from 67 adult (AHC) and 40 pediatric (PHC) healthy controls. DNA was isolated and analyzed using real-time PCR (Q-PCR) to determine the viral loads of MCPyV, LPV, JCV and BKV.
Results: MCPyV DNA was more frequently detected (p<0.05) in the PTP (36.9%) and PHC (30.0%) groups compared to JCV (8.7% in PTP, 12.5% in PHC), BKV (6.5% in PTP, 2.5% in PHC), and LPV (2.2% in PTP, 5% in PHC) and in the AHC (47.8%) group compared to BKV (13.4%) and LPV (0%).
Conclusions: Based on the results, it could be concluded that: (a) Despite the rarity of MCC, MCPyV is a common infection; (b) MCPyV demonstrates an excretion pattern similar to those of JCV and BKV, persisting in the kidney and infecting skin cells upon reactivation, with subsequent integration and transformation.
(Copyright © 2014 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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