Platelet rich plasma enhances the immunohistochemical expression of platelet derived growth factor and vascular endothelial growth factor in the synovium of the meniscectomized rat models of osteoarthritis.

Autor: Almasry SM; Department of Anatomy, Taibah University, Almadinah Almonawarah, Saudi Arabia; Department of Anatomy and Embryology, Almansoura University, Almansoura, Egypt. Electronic address: shaima.masry@yahoo.com., Soliman HM; Department of Anatomy, Taibah University, Almadinah Almonawarah, Saudi Arabia; Department of Histology and Cell Biology, Zagazig University, Zagazig, Egypt., El-Tarhouny SA; Department of Biochemistry and Molecular Medicine, Taibah University, Almadinah Almonawarah, Saudi Arabia; Department of Medical Biochemistry, Zagazig University, Zagazig, Egypt., Algaidi SA; Department of Anatomy, Taibah University, Almadinah Almonawarah, Saudi Arabia., Ragab EM; Department of Orthopedic Surgery, Al Azhar University, Assuit Branch, Cairo, Egypt.
Jazyk: angličtina
Zdroj: Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft [Ann Anat] 2015 Jan; Vol. 197, pp. 38-49. Date of Electronic Publication: 2014 Nov 04.
DOI: 10.1016/j.aanat.2014.10.006
Abstrakt: This study was carried out on a rat model of surgically-induced osteoarthritis (OA) to assess the histological and immunohistochemical changes in the synovial membrane and to evaluate the effects of intra-articular injection of platelet rich plasma (PRP) in such cases. Forty five male albino rats were divided into 3 equal groups; control, surgically-induced OA and surgically-induced OA followed by intra-articular injection of PRP. Knee joints were processed for histological and immunohistochemical staining with anti-platelet derived growth factor (PDGF-A) and anti-vascular endothelial growth factor (VEGF) and the area percentages of immunostaining were measured by digital image analysis. Serum levels of PDGF-A and VEGF were analyzed by ELISA. The osteoarthritis research society international (OARSI) score was significantly higher in OA (2433.8±254) than in control (230.4±37.8; p<0.001) and in PRP-treated tissues (759.7±45.8; p<0.001). The immunostained area percentages for PDGF-A was significantly higher in PRP-treated tissues (20.6±2.4) than in OA (11.06±1.3; p=0.007) and in control tissues (4.1±0.78; p<0.001). Likewise, the immunostained area percentage for VEGF was significantly higher in PRP-treated tissues (22.5±1.6) than in OA (14.9±1; p=0. 002) and in control tissues (6.5±0.7; p<0.001). ELISA analysis revealed a significant increase in serum levels of the PDGF-A and VEGF after intraarticular PRP injection when compared to the other groups (p<0.000). The present study concluded that intra-articular injection of PRP could produce optimizing effects in surgically induced OA in the form of; decreasing the OARSI score, improving the inflammatory events in synovium and modulating the PDGF-A and VEGF serum levels and synovial tissue immunoexpression. These effects could be reflected positively on the associated chondral defect.
(Copyright © 2014 Elsevier GmbH. All rights reserved.)
Databáze: MEDLINE
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