Biodegradation of tributyl phosphate, an organosphate triester, by aerobic granular biofilms.

Autor: Nancharaiah YV; Biofouling and Biofilm Processes Section, Water and Steam Chemistry Division, Chemistry Group, Bhabha Atomic Research Centre, Kalpakkam 603102, Tamil Nadu, India. Electronic address: venkatany@gmail.com., Kiran Kumar Reddy G; Biofouling and Biofilm Processes Section, Water and Steam Chemistry Division, Chemistry Group, Bhabha Atomic Research Centre, Kalpakkam 603102, Tamil Nadu, India., Krishna Mohan TV; Biofouling and Biofilm Processes Section, Water and Steam Chemistry Division, Chemistry Group, Bhabha Atomic Research Centre, Kalpakkam 603102, Tamil Nadu, India., Venugopalan VP; Biofouling and Biofilm Processes Section, Water and Steam Chemistry Division, Chemistry Group, Bhabha Atomic Research Centre, Kalpakkam 603102, Tamil Nadu, India.
Jazyk: angličtina
Zdroj: Journal of hazardous materials [J Hazard Mater] 2015; Vol. 283, pp. 705-11. Date of Electronic Publication: 2014 Oct 17.
DOI: 10.1016/j.jhazmat.2014.09.065
Abstrakt: Tributyl phosphate (TBP) is commercially used in large volumes for reprocessing of spent nuclear fuel. TBP is a very stable compound and persistent in natural environments and it is not removed in conventional wastewater treatment plants. In this study, cultivation of aerobic granular biofilms in a sequencing batch reactor was investigated for efficient biodegradation of TBP. Enrichment of TBP-degrading strains resulted in efficient degradation of TBP as sole carbon or along with acetate. Complete biodegradation of 2mM of TBP was achieved within 5h with a degradation rate of 0.4 μmol mL(-1) h(-1). TBP biodegradation was accompanied by release of inorganic phosphate in stoichiometric amounts. n-Butanol, hydrolysed product of TBP was rapidly biodegraded. But, dibutyl phosphate, a putative intermediate of TBP degradation was only partially degraded pointing to an alternative degradation pathway. Phosphatase activity was 22- and 7.5-fold higher in TBP-degrading biofilms as compared to bioflocs and acetate-fed aerobic granules. Community analysis by terminal restriction length polymorphism revealed presence of 30 different bacterial strains. Seven bacterial stains, including Sphingobium sp. a known TBP degrader were isolated. The results show that aerobic granular biofilms are promising for treatment of TBP-bearing wastes or ex situ bioremediation of TBP-contaminated sites.
(Copyright © 2014 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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