Tetracycline improved the efficiency of other antimicrobials against Gram-negative multidrug-resistant bacteria.

Autor:	Mawabo IK; Department of Biochemistry, Faculty of Science, University of Dschang, Cameroon., Noumedem JA; Department of Biochemistry, Faculty of Science, University of Dschang, Cameroon., Kuiate JR; Department of Biochemistry, Faculty of Science, University of Dschang, Cameroon., Kuete V; Department of Biochemistry, Faculty of Science, University of Dschang, Cameroon. Electronic address: kuetevictor@yahoo.fr.
Jazyk:	angličtina
Zdroj:	Journal of infection and public health [J Infect Public Health] 2015 May-Jun; Vol. 8 (3), pp. 226-33. Date of Electronic Publication: 2014 Oct 27.
DOI:	10.1016/j.jiph.2014.09.001
Abstrakt:	Treatment of infectious diseases with antimicrobials constituted a great achievement in the history of medicine. Unfortunately, the emergence of resistant strains of bacteria to all classes of antimicrobials limited their efficacy. The present study was aimed at evaluating the effect of combinations of antibiotics on multi-drug resistant Gram-negative (MDRGN) bacteria. A liquid micro-broth dilution method was used to evaluate the antibacterial activity of 10 different classes of antimicrobials on 20 bacterial strains belonging to six different species. The antimicrobials were associated with phenylalanine β-naphthylamide (PAβN), an efflux pump inhibitor, and with other antimicrobials at their sub-inhibitory concentrations. The effectiveness of each combination was monitored using the minimal inhibitory concentration (MIC) and the fractional inhibitory concentration (FIC). Most of the antimicrobials tested showed low antibacterial activity with a MIC value of 128 mg/L on a majority of the bacterial strains, justifying their multidrug-resistant (MDR) profile. Synergistic effects were mostly observed (FIC≤0.5) when ampicillin (AMP), cloxacillin (CLX), erythromycin (ERY), chloramphenicol (CHL), kanamycin (KAN) and streptomycin (STR) were combined with tetracycline (TET) at the sub-inhibitory concentration of MIC/5 or MIC/10. The results of the present work suggest that the association of several antimicrobials with TET could improve the fight against MDRGN bacterial species. (Copyright © 2014 King Saud Bin Abdulaziz University for Health Sciences. Published by Elsevier Ltd. All rights reserved.)
Databáze:	MEDLINE
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