Toxicity assessment of aggregated/agglomerated cerium oxide nanoparticles in an in vitro 3D airway model: the influence of mucociliary clearance.

Autor: Frieke Kuper C; TNO, Utrechtseweg 48, 3704 HE Zeist, The Netherlands. Electronic address: frieke.kuper@tno.nl., Gröllers-Mulderij M; TNO, Utrechtseweg 48, 3704 HE Zeist, The Netherlands. Electronic address: mariska.grollers@tno.nl., Maarschalkerweerd T; TNO, Princetonlaan 6, 3584 CB Utrecht, The Netherlands. Electronic address: therese.maarschalkerweerd@tno.nl., Meulendijks NM; TNO, De Rondom 1, 5612 AP Eindhoven, The Netherlands. Electronic address: nicole.meulendijks@tno.nl., Reus A; TNO Triskelion bv, Utrechtseweg 48, 3704 HE Zeist, The Netherlands. Electronic address: astrid.reus@tno.triskelion.nl., van Acker F; TNO Triskelion bv, Utrechtseweg 48, 3704 HE Zeist, The Netherlands. Electronic address: frederique.vanacker@tno.triskelion.nl., Zondervan-van den Beuken EK; TNO, Utrechtseweg 48, 3704 HE Zeist, The Netherlands. Electronic address: esther.zondervan-vandenbeuken@tno.nl., Wouters ME; TNO, De Rondom 1, 5612 AP Eindhoven, The Netherlands. Electronic address: marielle.wouters@tno.nl., Bijlsma S; TNO, Utrechtseweg 48, 3704 HE Zeist, The Netherlands. Electronic address: sabina.bijlsma@tno.nl., Kooter IM; TNO, Princetonlaan 6, 3584 CB Utrecht, The Netherlands. Electronic address: ingeborg.kooter@tno.nl.
Jazyk: angličtina
Zdroj: Toxicology in vitro : an international journal published in association with BIBRA [Toxicol In Vitro] 2015 Mar; Vol. 29 (2), pp. 389-97. Date of Electronic Publication: 2014 Nov 04.
DOI: 10.1016/j.tiv.2014.10.017
Abstrakt: We investigated the toxicity of aggregated nanoparticles of cerium oxide (CeO2) using an in vitro 3D human bronchial epithelial model that included a mucociliary apparatus (MucilAir™). CeO2 was dispersed in saline and applied to the apical surface of the model. CeO2 did not induce distinct effects in the model, whereas it did in BEAS-2B and A549 cell cultures. The absence of effects of CeO2 was not because of the model's insensitivity. Nanoparticles of zinc oxide (ZnO) elicited positive responses in the toxicological assays. Respiratory mucus (0.1% and 1%) added to dispersions increased aggregation/agglomeration to such an extent that most CeO2 sedimented within a few minutes. Also, the mucociliary apparatus of the model removed CeO2 from the central part of the apical surface to the borders. This 'clearance' may have prevented the majority of CeO2 from reaching the epithelial cells. Chemical analysis of cerium in the basal tissue culture medium showed only minimal translocation of cerium across the 3D barrier. In conclusion, mucociliary defence appeared to prevent CeO2 reaching the respiratory epithelial cells in this 3D in vitro model. This model and approach can be used to study compounds of specific toxicological concern in airway defence mechanisms in vitro.
(Copyright © 2014 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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