Saccharomyces cerevisiae SHSY detoxifies petroleum n-alkanes by an induced CYP52A58 and an enhanced order in cell surface hydrophobicity.

Autor: Hanano A; Department of Molecular Biology and Biotechnology, Atomic Energy Commission of Syria (AECS), B.P. Box 6091, Damascus, Syria. Electronic address: ashanano@aec.org.sy., Shaban M; Department of Molecular Biology and Biotechnology, Atomic Energy Commission of Syria (AECS), B.P. Box 6091, Damascus, Syria., Almousally I; Department of Molecular Biology and Biotechnology, Atomic Energy Commission of Syria (AECS), B.P. Box 6091, Damascus, Syria., Al-Ktaifani M; Department of Chemistry, Atomic Energy Commission of Syria (AECS), B.P. Box 6091, Damascus, Syria.
Jazyk: angličtina
Zdroj: Chemosphere [Chemosphere] 2015 Sep; Vol. 135, pp. 418-26. Date of Electronic Publication: 2014 Nov 27.
DOI: 10.1016/j.chemosphere.2014.11.011
Abstrakt: Environmental hydrocarbon contamination has a serious hazard to human health. Alkanes, the major component of hydrocarbons, can be consumed by various species of yeast. We previously identified a new strain SHSY of Saccharomyces cerevisiae with a remarkable ability to utilize the petroleum crude-oil (PCO) in aqueous solution. The current study demonstrated that the n-alkanes-assimilation activity of S. cerevisiae SHSY was related to an induced microsomal protein of 59 kDa approximately. The identified ORF encoded a protein of 517 amino acids and shared 93% sequence identity with an alkane-inducible hydroxylase CYP52A53 isolated from Scheffersomyces stipitis CBS. It was therefore referred as CYP52A58. The catalytic activity of the recombinant CYP52A58 was confirmed by the hydroxylation of n-alkanes, it showed an optimal mono-terminal hydroxylation activity toward n-hexadecane. Moreover, the ability of the yeast to use n-alkanes was accompanied with an increasing level in cell wall mannoproteins. Two differential protein bands were detected in the mannoproteins extracted from PCO-grown yeast. In parallel, a significant increase in the fatty acids content with a high degree of unsaturation was subsequently detected in the PCO-grown yeast. This study characterizes a safe and potential microorganism to remove n-alkanes from the aquatic environment.
(Copyright © 2014 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: