| Autor: |
Ottenheim C; Microbiology and Bioprocess Engineering, Institute of Biochemistry, Leipzig University, 04103, Leipzig, Germany, christoph@ottenheim.net., Meier K, Zimmermann W, Wu JC |
| Jazyk: |
angličtina |
| Zdroj: |
Applied biochemistry and biotechnology [Appl Biochem Biotechnol] 2015 Feb; Vol. 175 (4), pp. 2066-74. Date of Electronic Publication: 2014 Nov 30. |
| DOI: |
10.1007/s12010-014-1427-8 |
| Abstrakt: |
For complete degradation of hemicellulose into its monomers from lignocellulose biomass, the synergistic action of a broad range of hydrolytic enzymes is needed. Therefore, production of enzymes from their natural producer is desirable. To obtain a powerful β-1,4-endoxylanase producing fungus, 304 environmental samples were collected from various locations in Singapore, leading to 603 isolates. Among them, 71 exhibiting β-1,4-endoxylanase activity were identified belonging mainly to the genera of Aspergillus, Penicillium, and Trichoderma. Further analysis revealed Aspergillus niger DSM 26641 as a potential and stable β-1,4-endoxylanase producer, being able to grow in hydrothermal lignocellulose hydrolysate exhibiting its maximal β-1,4-endoxylanase activity at pH 4 and 60 °C. This strain is thought to be very suitable for lactic acid production in a simultaneous saccharification and fermentation at pH values below 5. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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