| Autor: |
Fry AL; Center for Cell Biology and Cancer Research, Albany Medical College, Albany, New York 12208, USA., Laboy JT; Center for Cell Biology and Cancer Research, Albany Medical College, Albany, New York 12208, USA., Norman KR; Center for Cell Biology and Cancer Research, Albany Medical College, Albany, New York 12208, USA. |
| Jazyk: |
angličtina |
| Zdroj: |
Nature communications [Nat Commun] 2014 Nov 21; Vol. 5, pp. 5579. Date of Electronic Publication: 2014 Nov 21. |
| DOI: |
10.1038/ncomms6579 |
| Abstrakt: |
The complex molecular and cellular mechanisms underlying neuronal control of animal movement are not well understood. Locomotion of Caenorhabditis elegans is mediated by a neuronal circuit that produces coordinated sinusoidal movement. Here we utilize this simple, yet elegant, behaviour to show that VAV-1, a conserved guanine nucleotide exchange factor for Rho-family GTPases, negatively regulates motor circuit activity and the rate of locomotion. While vav-1 is expressed in a small subset of neurons, we find that VAV-1 function is required in a single interneuron, ALA, to regulate motor neuron circuit activity. Furthermore, we show by genetic and optogenetic manipulation of ALA that VAV-1 is required for the excitation and activation of this neuron. We find that ALA signalling inhibits command interneuron activity by abrogating excitatory signalling in the command interneurons, which is responsible for promoting motor neuron circuit activity. Together, our data describe a novel neuromodulatory role for VAV-1-dependent signalling in the regulation of motor circuit activity and locomotion. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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