| Autor: |
Leyn SA; A.A. Kharkevich Institute for Information Transmission Problems, Russian Academy of Sciences, Moscow, Russia., Suvorova IA; A.A. Kharkevich Institute for Information Transmission Problems, Russian Academy of Sciences, Moscow, Russia., Kholina TD; Faculty of Bioengineering and Bioinformatics, Moscow State University, Moscow, Russia., Sherstneva SS; Comprehensive School 463, Moscow, Russia., Novichkov PS; Lawrence Berkeley National Laboratory, Berkeley, California, United States of America., Gelfand MS; A.A. Kharkevich Institute for Information Transmission Problems, Russian Academy of Sciences, Moscow, Russia; Faculty of Bioengineering and Bioinformatics, Moscow State University, Moscow, Russia., Rodionov DA; A.A. Kharkevich Institute for Information Transmission Problems, Russian Academy of Sciences, Moscow, Russia; Sanford-Burnham Medical Research Institute, La Jolla, California, United States of America. |
| Abstrakt: |
Methionine metabolism and uptake genes in Proteobacteria are controlled by a variety of RNA and DNA regulatory systems. We have applied comparative genomics to reconstruct regulons for three known transcription factors, MetJ, MetR, and SahR, and three known riboswitch motifs, SAH, SAM-SAH, and SAM_alpha, in ∼ 200 genomes from 22 taxonomic groups of Proteobacteria. We also identified two novel regulons: a SahR-like transcription factor SamR controlling various methionine biosynthesis genes in the Xanthomonadales group, and a potential RNA regulatory element with terminator-antiterminator mechanism controlling the metX or metZ genes in beta-proteobacteria. For each analyzed regulator we identified the core, taxon-specific and genome-specific regulon members. By analyzing the distribution of these regulators in bacterial genomes and by comparing their regulon contents we elucidated possible evolutionary scenarios for the regulation of the methionine metabolism genes in Proteobacteria. |
