Characterization of two strains of Tribeč virus isolated in Ukraine.

Autor: Dedkov VG; 1 Central Research Institute for Epidemiology , Russian Inspectorate for Protection of Consumer Rights and Human Welfare, Moscow, Russia ., Dubina DA, Yurchenko OA, Bekova MV, Valdokhina AV, Shipulin GA
Jazyk: angličtina
Zdroj: Vector borne and zoonotic diseases (Larchmont, N.Y.) [Vector Borne Zoonotic Dis] 2014 Nov; Vol. 14 (11), pp. 808-16.
DOI: 10.1089/vbz.2014.1683
Abstrakt: During arbovirus surveillance in Ixodes ricinus ticks in South Ukraine, two strains of Tribeč virus were isolated using an in vivo method and characterized using the complement fixation text (CFT), hemagglutination assay (HA), electron microscopy, and molecular methods. Both strains replicated well in the BHK-21, PEC, and Vero cell lines and demonstrated 95% nucleotide identity in their VP3 sequences compared with the reference VP3 sequence for Tribeč virus. These two strains of Tribeč virus were named Tr35 and Tr19. Also, segment reassortment involving Tr35, Tr19, TRBV, and LIPV strains was shown.
Databáze: MEDLINE