Mammalian target of rapamycin complex 2 regulates invariant NKT cell development and function independent of promyelocytic leukemia zinc-finger.

Autor:	Prevot N; Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109;, Pyaram K; Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109;, Bischoff E; Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109;, Sen JM; Laboratory of Molecular Biology and Immunology, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224; and., Powell JD; Department of Oncology, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD 21231., Chang CH; Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109; heechang@umich.edu.
Jazyk:	angličtina
Zdroj:	Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2015 Jan 01; Vol. 194 (1), pp. 223-30. Date of Electronic Publication: 2014 Nov 17.
DOI:	10.4049/jimmunol.1401985
Abstrakt:	The mammalian target of rapamycin (mTOR) senses and incorporates different environmental cues via the two signaling complexes mTOR complex 1 (mTORC1) and mTORC2. As a result, mTOR controls cell growth and survival, and also shapes different effector functions of the cells including immune cells such as T cells. We demonstrate in this article that invariant NKT (iNKT) cell development is controlled by mTORC2 in a cell-intrinsic manner. In mice deficient in mTORC2 signaling because of the conditional deletion of the Rictor gene, iNKT cell numbers were reduced in the thymus and periphery. This is caused by decreased proliferation of stage 1 iNKT cells and poor development through subsequent stages. Functionally, iNKT cells devoid of mTORC2 signaling showed reduced number of IL-4-expressing cells, which correlated with a decrease in the transcription factor GATA-3-expressing cells. However, promyelocytic leukemia zinc-finger (PLZF), a critical transcription factor for iNKT cell development, is expressed at a similar level in mTORC2-deficient iNKT cells compared with that in the wild type iNKT cells. Furthermore, cellular localization of PLZF was not altered in the absence of mTOR2 signaling. Thus, our study reveals the PLZF-independent mechanisms of the development and function of iNKT cells regulated by mTORC2. (Copyright © 2014 by The American Association of Immunologists, Inc.)
Databáze:	MEDLINE
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