Development of a sensitive ultra high performance liquid chromatography with tandem mass spectrometry method for the simultaneous quantification of nine active compounds in rat plasma and its application to a pharmacokinetic study after administration of Viscum coloratum extracts.
Autor: | Fan RH; School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China., Ding W, Ma YY, Lin HL, Men L, Duan MM, Zhao YL, Yu ZG |
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Jazyk: | angličtina |
Zdroj: | Journal of separation science [J Sep Sci] 2015 Feb; Vol. 38 (3), pp. 530-40. Date of Electronic Publication: 2014 Dec 23. |
DOI: | 10.1002/jssc.201400804 |
Abstrakt: | A simple, specific, and sensitive ultra high performance liquid chromatography with tandem mass spectrometry method was developed and validated for the simultaneous quantification of nine compounds including a new compound, rhamnazin-3-Ο-β-D-(6″-β-hydroxy-β-methyglutaryl)-β-D-glucoside-4'-Ο-β-D-glucoside, in rat plasma using baicalin as an internal standard. The plasma samples were pretreated and extracted by protein precipitation with 0.2% formic acid in acetonitrile. The analytes were separated on a Thermo Syncronis C18 column by gradient elution with a mobile phase consisting of acetonitrile and 0.1% aqueous formic acid at a flow rate of 0.25 mL/min. The detection of the analytes was performed on an electrospray ionization interface operating in positive-ion and multiple reaction monitoring acquisition modes. The calibration curves of these analytes showed good linearity (r > 0.99) within the test ranges. The lower limit of quantification ranged from 0.4 to 20.1 ng/mL for the analytes. The intra- and interday precision and accuracy were all within ±15%, and the recoveries were higher than 80.0%. The validated method was successfully applied to a pharmacokinetic study of the nine flavonoids after administration of the Viscum coloratum extracts by intravenous injection. (© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.) |
Databáze: | MEDLINE |
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