| Autor: |
de Araujo Filho HB; Division of Pediatric Gastroenterology, Universidade Federal de São Paulo, 598 Botucatu Street, Vila Clementino, 04023-062 São Paulo, SP, Brazil ; Centro Universitário FIEO, 300 Franz Voegeli Avenida, Vila Yara, 06020-190 Osasco, SP, Brazil., Carmo-Rodrigues MS; Centro Universitário FIEO, 300 Franz Voegeli Avenida, Vila Yara, 06020-190 Osasco, SP, Brazil., Mello CS; Division of Pediatric Gastroenterology, Universidade Federal de São Paulo, 598 Botucatu Street, Vila Clementino, 04023-062 São Paulo, SP, Brazil., Melli LC; Division of Pediatric Gastroenterology, Universidade Federal de São Paulo, 598 Botucatu Street, Vila Clementino, 04023-062 São Paulo, SP, Brazil ; Centro Universitário FIEO, 300 Franz Voegeli Avenida, Vila Yara, 06020-190 Osasco, SP, Brazil., Tahan S; Division of Pediatric Gastroenterology, Universidade Federal de São Paulo, 598 Botucatu Street, Vila Clementino, 04023-062 São Paulo, SP, Brazil., Pignatari AC; Division of Medicine, Universidade Federal de São Paulo, 188 Leandro Dupret Street, Vila Clementino, 04025-010 São Paulo, SP, Brazil., de Morais MB; Division of Pediatric Gastroenterology, Universidade Federal de São Paulo, 598 Botucatu Street, Vila Clementino, 04023-062 São Paulo, SP, Brazil. |
| Abstrakt: |
This study evaluated the breath CH4 excretion and concentration of M. smithii in intestinal microbiota of schoolchildren from 2 slums. One hundred and eleven children from a slum near a sanitary landfill, 35 children of a slum located away from the sanitary landfill, and 32 children from a high socioeconomic level school were included in the study. Real-time PCR was performed to quantify the M. smithii nifH gene and it was present in the microbiota of all the participating children, with higher (P < 0.05) concentrations in those who lived in the slum near the landfill (3.16 × 10(7) CFU/g of feces), comparing with the children from the slum away from the landfill (2.05 × 10(6) CFU/g of feces) and those from the high socioeconomic level group (3.93 × 10(5) CFU/g of feces). The prevalence of children who present breath methane was 53% in the slum near the landfill, 31% in the slum further away from the landfill and, 22% in the high socioeconomic level group. To live near a landfill is associated with higher concentrations of M. smithii in intestinal microbiota, comparing with those who live away from the landfill, regardless of their socioeconomics conditions. |
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