[Human caspase-14 expression in malignant melanoma and its significance].

Autor:	Wang X; Department of Pathophysiology, School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China., Zhang J; Department of Forensic Genetics, School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China., Cheng Y; Department of Pathophysiology, School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China., DU J; Department of Forensic Genetics, School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China., Chen D; Department of Pathophysiology, School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China., Yang Z; Department of Pathophysiology, School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China., Wang Y; Department of Pathophysiology, School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China., Huang N; Department of Pathophysiology, School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Jazyk:	čínština
Zdroj:	Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology [Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi] 2014 Nov; Vol. 30 (11), pp. 1180-3.
Abstrakt:	Objective: To detect the caspase-14 expression in malignant melanoma cells and tumor tissues and its effect on tumor resistance to drug. Methods: The mRNA and protein level of caspase-14 in 4 melanoma cell lines (A375, A875, M14, and SK-Mel-1) and the melanocytes, was detected by reverse transcription PCR (RT-PCR) and Western blotting. Caspase-14 expression in 34 malignant melanoma tumor tissues and 10 dermal nevus tissues was determined by in situ hybridization and immunohistochemistry. Results: Caspase-14 expression was seen in melanoma cells and melanocytes. It was higher in melanoma-associated antigen 1 recognized by T cells (MART-1) positive cells than in MART-1 negative cells. The cells expressing the lower caspase-14 were more sensitive to the treatment with either chemotherapy drugs camptothecin and cisplatin or radiotherapy than the ones expressing the higher caspase-14 (P<0.01). Caspase-14 expression was observed in 70% dermal nevus, as well as 97% in malignant melanoma tissues, and the difference between them was statistically significant (P<0.05). Conclusion: Caspase-14 is expressed in tissues and cells of malignant melanoma. Our data indicated that the expression level of caspase-14 affected the drug sensitivity of melanoma.
Databáze:	MEDLINE
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