| Autor: |
Shinoda A; Graduate School of Life Science, Hokkaido University, Sapporo, Hokkaido 060-0810, Japan., Tanaka Y; Graduate School of Life Science, Hokkaido University, Sapporo, Hokkaido 060-0810, Japan., Yao M; Graduate School of Life Science, Hokkaido University, Sapporo, Hokkaido 060-0810, Japan., Tanaka I; Faculty of Advanced Life Science, Hokkaido University, Sapporo, Hokkaido 060-0810, Japan. |
| Abstrakt: |
X-ray crystallography is an important technique for structure-based drug discovery, mainly because it is the only technique that can reveal whether a ligand binds to the target protein as well as where and how it binds. However, ligand screening by X-ray crystallography involves a crystal-soaking experiment, which is usually performed manually. Thus, the throughput is not satisfactory for screening large numbers of candidate ligands. In this study, a technique to anchor protein crystals to mounting loops by using gel and inkjet technology has been developed; the method allows soaking of the mounted crystals in ligand-containing solution. This new technique may assist in the design of a fully automated drug-screening pipeline. |
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