Using in-gel digestion and an Orbitrap mass spectrometer to analyze the proteome of clathrin-coated vesicles.
| Autor: | Borner GH; Department of Proteomics and Signal Transduction, Max Plank Institute of Biochemistry, 82152 Martinsried, Germany;, Fielding AB; Department of Cellular and Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Liverpool L69 3BX, United Kingdom. |
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| Jazyk: | angličtina |
| Zdroj: | Cold Spring Harbor protocols [Cold Spring Harb Protoc] 2014 Nov 03; Vol. 2014 (11), pp. 1188-91. Date of Electronic Publication: 2014 Nov 03. |
| DOI: | 10.1101/pdb.prot083154 |
| Abstrakt: | The characterization of clathrin-coated vesicles (CCVs), including the effects of genetic or biochemical manipulations on their composition, can be studied by mass spectrometry analysis of HeLa cell fractions enriched for CCVs. This protocol describes the preparation of samples by tryptic in-gel digest and peptide extraction followed by analysis in an Orbitrap mass spectrometer. (© 2014 Cold Spring Harbor Laboratory Press.) |
| Databáze: | MEDLINE |
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