Isolating HeLa cell fractions enriched for clathrin-coated vesicles.
| Autor: | Borner GH; Department of Proteomics and Signal Transduction, Max Plank Institute of Biochemistry, 82152 Martinsried, Germany;, Fielding AB; Department of Cellular and Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Liverpool L69 3BX, United Kingdom. |
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| Jazyk: | angličtina |
| Zdroj: | Cold Spring Harbor protocols [Cold Spring Harb Protoc] 2014 Nov 03; Vol. 2014 (11), pp. 1184-7. Date of Electronic Publication: 2014 Nov 03. |
| DOI: | 10.1101/pdb.prot083147 |
| Abstrakt: | HeLa cell lines can be experimentally manipulated using drugs or gene-silencing techniques such as RNA interference. Fractions enriched for clathrin-coated vesicles (CCVs) can be isolated from these cell lines and used to study the effects of these manipulations on the composition of CCVs. This protocol, originally developed in the laboratory of Margaret Robinson (Cambridge, United Kingdom), describes the preparation of a HeLa cell fraction that is enriched for a mixed population of CCVs and is suitable for analysis by mass spectroscopy, western blotting, or electron microscopy. (© 2014 Cold Spring Harbor Laboratory Press.) |
| Databáze: | MEDLINE |
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