| Autor: |
de Cassia Pereira J; Laboratory of Biochemistry and Applied Microbiology, Universidade Estadual Paulista (UNESP), Av. Cristóvão Colombo, 2265, 15054-000, São José do Rio Preto, São Paulo, Brazil, josianipereira@gmail.com., Leite RS, do Prado HF, Bocchini Martins DA, Gomes E, da Silva R |
| Jazyk: |
angličtina |
| Zdroj: |
Applied biochemistry and biotechnology [Appl Biochem Biotechnol] 2015 Jan; Vol. 175 (2), pp. 723-32. Date of Electronic Publication: 2014 Oct 24. |
| DOI: |
10.1007/s12010-014-1332-1 |
| Abstrakt: |
In this paper, several agro-industrial wastes (soybean meal and wheat straw, rice and peanut husks, corn cob and corn stover, and sugarcane bagasse) were tested for the production of β-glucosidase by the cultivation of thermophilic fungus Thermomucor indicae-seudaticae N31 in solid-state fermentation (SSF). Among the tested substrates, the highest yields were obtained in soybean meal. Other fermentation parameters were also evaluated, such as initial pH, merge substrates, and fermentation time, as well as the physicochemical characterization of the enzyme. The best results were obtained after 192 h of fermentation with the initial pH adjusted to 6.0. The substrate mixture did not improve the enzyme production by microorganism. The β-glucosidase showed best catalytic activity at pH 4.5 and at 75 °C and remained stable in the pH range from 4.5 to 9.5 and the temperature range 40-75 °C. The enzyme showed 80 % of its activity at a concentration of 15 mM glucose and remained stable up to 20 % ethanol. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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