Acylcarnitines: potential implications for skeletal muscle insulin resistance.

Autor: Aguer C; Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, Ontario, Canada;, McCoin CS; Molecular, Cellular, & Integrative Physiology Graduate Program, University of California, Davis, California, USA; Obesity & Metabolism Research Unit, United States Department of Agriculture-Agricultural Research Service Western Human Nutrition Research Center, Davis, California, USA;, Knotts TA; Obesity & Metabolism Research Unit, United States Department of Agriculture-Agricultural Research Service Western Human Nutrition Research Center, Davis, California, USA; Department of Nutrition, University of California, Davis, California, USA;, Thrush AB; Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, Ontario, Canada;, Ono-Moore K; Obesity & Metabolism Research Unit, United States Department of Agriculture-Agricultural Research Service Western Human Nutrition Research Center, Davis, California, USA; Department of Nutrition, University of California, Davis, California, USA;, McPherson R; Division of Cardiology, University of Ottawa Heart Institute, Ottawa, Ontario, Canada;, Dent R; Ottawa Hospital Weight Management Clinic, Ottawa, Ontario, Canada;, Hwang DH; Immunity & Disease Prevention Research Unit, U.S. Department of Agriculture-Agricultural Research Service Western Human Nutrition Research Center, Davis, California, USA Department of Nutrition, University of California, Davis, California, USA;, Adams SH; Molecular, Cellular, & Integrative Physiology Graduate Program, University of California, Davis, California, USA; Obesity & Metabolism Research Unit, United States Department of Agriculture-Agricultural Research Service Western Human Nutrition Research Center, Davis, California, USA; Department of Nutrition, University of California, Davis, California, USA; mharper@uottawa.ca seanharrisonadams@gmail.com., Harper ME; Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, Ontario, Canada; mharper@uottawa.ca seanharrisonadams@gmail.com.
Jazyk: angličtina
Zdroj: FASEB journal : official publication of the Federation of American Societies for Experimental Biology [FASEB J] 2015 Jan; Vol. 29 (1), pp. 336-45. Date of Electronic Publication: 2014 Oct 23.
DOI: 10.1096/fj.14-255901
Abstrakt: Insulin resistance may be linked to incomplete fatty acid β-oxidation and the subsequent increase in acylcarnitine species in different tissues including skeletal muscle. It is not known if acylcarnitines participate in muscle insulin resistance or simply reflect dysregulated metabolism. The aims of this study were to determine whether acylcarnitines can elicit muscle insulin resistance and to better understand the link between incomplete muscle fatty acid β-oxidation, oxidative stress, inflammation, and insulin-resistance development. Differentiated C2C12, primary mouse, and human myotubes were treated with acylcarnitines (C4:0, C14:0, C16:0) or with palmitate with or without carnitine acyltransferase inhibition by mildronate. Treatment with C4:0, C14:0, and C16:0 acylcarnitines resulted in 20-30% decrease in insulin response at the level of Akt phosphorylation and/or glucose uptake. Mildronate reversed palmitate-induced insulin resistance concomitant with an ∼25% decrease in short-chain acylcarnitine and acetylcarnitine secretion. Although proinflammatory cytokines were not affected under these conditions, oxidative stress was increased by 2-3 times by short- or long-chain acylcarnitines. Acylcarnitine-induced oxidative stress and insulin resistance were reversed by treatment with antioxidants. Results are consistent with the conclusion that incomplete muscle fatty acid β-oxidation causes acylcarnitine accumulation and associated oxidative stress, raising the possibility that these metabolites play a role in muscle insulin resistance.
(© FASEB.)
Databáze: MEDLINE
Externí odkaz: