Origin and ecological selection of core and food-specific bacterial communities associated with meat and seafood spoilage.

Autor: Chaillou S; 1] INRA, UMR1319 Micalis, Lactic Acid Bacteria & Meat Microbial Ecosystems Laboratory, Paris, France [2] AgroParisTech, UMR Micalis, Paris, France., Chaulot-Talmon A; 1] INRA, UMR1319 Micalis, Lactic Acid Bacteria & Meat Microbial Ecosystems Laboratory, Paris, France [2] AgroParisTech, UMR Micalis, Paris, France., Caekebeke H; Plate-Forme d'Innovations Nouvelles Vagues, Boulogne-sur-mer, France., Cardinal M; Ifremer, Nantes, France., Christieans S; ADIV, Clermont-Ferrand, France., Denis C; ACTALIA, Villers Bocage, France., Desmonts MH; Aérial, Strasbourg, France., Dousset X; 1] INRA, UMR1014 Secalim, Nantes, France [2] LUNAM Université, Oniris, Nantes, France., Feurer C; IFIP, Paris, France., Hamon E; Aérial, Strasbourg, France., Joffraud JJ; Ifremer, Nantes, France., La Carbona S; ACTALIA, Villers Bocage, France., Leroi F; Ifremer, Nantes, France., Leroy S; INRA, UR454 Microbiologie, Saint-Genès-Champanelle, France., Lorre S; Biocéane, Nantes, France., Macé S; 1] INRA, UMR1014 Secalim, Nantes, France [2] LUNAM Université, Oniris, Nantes, France., Pilet MF; 1] INRA, UMR1014 Secalim, Nantes, France [2] LUNAM Université, Oniris, Nantes, France., Prévost H; 1] INRA, UMR1014 Secalim, Nantes, France [2] LUNAM Université, Oniris, Nantes, France., Rivollier M; ADIV, Clermont-Ferrand, France., Roux D; Plate-Forme d'Innovations Nouvelles Vagues, Boulogne-sur-mer, France., Talon R; INRA, UR454 Microbiologie, Saint-Genès-Champanelle, France., Zagorec M; 1] INRA, UMR1014 Secalim, Nantes, France [2] LUNAM Université, Oniris, Nantes, France., Champomier-Vergès MC; 1] INRA, UMR1319 Micalis, Lactic Acid Bacteria & Meat Microbial Ecosystems Laboratory, Paris, France [2] AgroParisTech, UMR Micalis, Paris, France.
Jazyk: angličtina
Zdroj: The ISME journal [ISME J] 2015 May; Vol. 9 (5), pp. 1105-18. Date of Electronic Publication: 2014 Oct 21.
DOI: 10.1038/ismej.2014.202
Abstrakt: The microbial spoilage of meat and seafood products with short shelf lives is responsible for a significant amount of food waste. Food spoilage is a very heterogeneous process, involving the growth of various, poorly characterized bacterial communities. In this study, we conducted 16S ribosomal RNA gene pyrosequencing on 160 samples of fresh and spoiled foods to comparatively explore the bacterial communities associated with four meat products and four seafood products that are among the most consumed food items in Europe. We show that fresh products are contaminated in part by a microbiota similar to that found on the skin and in the gut of animals. However, this animal-derived microbiota was less prevalent and less abundant than a core microbiota, psychrotrophic in nature, mainly originated from the environment (water reservoirs). We clearly show that this core community found on meat and seafood products is the main reservoir of spoilage bacteria. We also show that storage conditions exert strong selective pressure on the initial microbiota: alpha diversity in fresh samples was 189±58 operational taxonomic units (OTUs) but dropped to 27±12 OTUs in spoiled samples. The OTU assemblage associated with spoilage was shaped by low storage temperatures, packaging and the nutritional value of the food matrix itself. These factors presumably act in tandem without any hierarchical pattern. Most notably, we were also able to identify putative new clades of dominant, previously undescribed bacteria occurring on spoiled seafood, a finding that emphasizes the importance of using culture-independent methods when studying food microbiota.
Databáze: MEDLINE
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