Alk3 mediated Bmp signaling controls the contribution of epicardially derived cells to the tissues of the atrioventricular junction.

Autor: Lockhart MM; Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina, Charleston, SC, USA., Boukens BJ; Department of Biomedical Engineering, Washington University, St. Louis, MO, USA., Phelps AL; Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina, Charleston, SC, USA., Brown CL; Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina, Charleston, SC, USA., Toomer KA; Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina, Charleston, SC, USA., Burns TA; Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina, Charleston, SC, USA., Mukherjee RD; Division of Cardiothoracic Surgery, Department of Surgery and Pediatrics, Medical University of South Carolina, Charleston, SC, USA., Norris RA; Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina, Charleston, SC, USA., Trusk TC; Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina, Charleston, SC, USA., van den Hoff MJ; Heart Failure Research Center, Department of Anatomy, Embryology and Physiology, Academic Medical Center, Amsterdam, The Netherlands., Wessels A; Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina, Charleston, SC, USA. Electronic address: wesselsa@musc.edu.
Jazyk: angličtina
Zdroj: Developmental biology [Dev Biol] 2014 Dec 01; Vol. 396 (1), pp. 8-18. Date of Electronic Publication: 2014 Oct 06.
DOI: 10.1016/j.ydbio.2014.09.031
Abstrakt: Recent studies using mouse models for cell fate tracing of epicardial derived cells (EPDCs) have demonstrated that at the atrioventricular (AV) junction EPDCs contribute to the mesenchyme of the AV sulcus, the annulus fibrosus, and the parietal leaflets of the AV valves. There is little insight, however, into the mechanisms that govern the contribution of EPDCs to these tissues. While it has been demonstrated that bone morphogenetic protein (Bmp) signaling is required for AV cushion formation, its role in regulating EPDC contribution to the AV junction remains unexplored. To determine the role of Bmp signaling in the contribution of EPDCs to the AV junction, the Bmp receptor activin-like kinase 3 (Alk3; or Bmpr1a) was conditionally deleted in the epicardium and EPDCs using the mWt1/IRES/GFP-Cre (Wt1(Cre)) mouse. Embryonic Wt1(Cre);Alk3(fl/fl) specimens showed a significantly smaller AV sulcus and a severely underdeveloped annulus fibrosus. Electrophysiological analysis of adult Wt1(Cre);Alk3(fl/fl) mice showed, unexpectedly, no ventricular pre-excitation. Cell fate tracing revealed a significant decrease in the number of EPDCs within the parietal leaflets of the AV valves. Postnatal Wt1(Cre);Alk3(fl/fl) specimens showed myxomatous changes in the leaflets of the mitral valve. Together these observations indicate that Alk3 mediated Bmp signaling is important in the cascade of events that regulate the contribution of EPDCs to the AV sulcus, annulus fibrosus, and the parietal leaflets of the AV valves. Furthermore, this study shows that EPDCs do not only play a critical role in early developmental events at the AV junction, but that they also are important in the normal maturation of the AV valves.
(Copyright © 2014 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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