Methods to study the tumor microenvironment under controlled oxygen conditions.

Autor: Byrne MB; Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA; Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA., Leslie MT; Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA; Department of Pathobiology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA., Gaskins HR; Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA; Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA; Department of Pathobiology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. Electronic address: hgaskins@illinois.edu., Kenis PJA; Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA; Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. Electronic address: kenis@illinois.edu.
Jazyk: angličtina
Zdroj: Trends in biotechnology [Trends Biotechnol] 2014 Nov; Vol. 32 (11), pp. 556-563. Date of Electronic Publication: 2014 Oct 02.
DOI: 10.1016/j.tibtech.2014.09.006
Abstrakt: The tumor microenvironment (TME) is a complex heterogeneous assembly composed of a variety of cell types and physical features. One such feature, hypoxia, is associated with metabolic reprogramming, the epithelial-mesenchymal transition, and therapeutic resistance. Many questions remain regarding the effects of hypoxia on these outcomes; however, only a few experimental methods enable both precise control over oxygen concentration and real-time imaging of cell behavior. Recent efforts with microfluidic platforms offer a promising solution to these limitations. In this review, we discuss conventional methods and tools used to control oxygen concentration for cell studies, and then highlight recent advances in microfluidic-based approaches for controlling oxygen in engineered platforms.
(Copyright © 2014 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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