RNA sequencing of microglia and monocyte-derived macrophages from mice with experimental autoimmune encephalomyelitis illustrates a changing phenotype with disease course.

Autor: Lewis ND; Department of Immunology and Inflammation, Boehringer Ingelheim Pharmaceuticals Inc., Ridgefield, CT 06877-0368, USA., Hill JD; Department of Research Networking, Boehringer Ingelheim Pharmaceuticals Inc., Ridgefield, CT, 06877-0368, USA., Juchem KW; Department of Immunology and Inflammation, Boehringer Ingelheim Pharmaceuticals Inc., Ridgefield, CT 06877-0368, USA., Stefanopoulos DE; Department of Immunology and Inflammation, Boehringer Ingelheim Pharmaceuticals Inc., Ridgefield, CT 06877-0368, USA., Modis LK; Department of Immunology and Inflammation, Boehringer Ingelheim Pharmaceuticals Inc., Ridgefield, CT 06877-0368, USA. Electronic address: ymodis@gmail.com.
Jazyk: angličtina
Zdroj: Journal of neuroimmunology [J Neuroimmunol] 2014 Dec 15; Vol. 277 (1-2), pp. 26-38. Date of Electronic Publication: 2014 Sep 20.
DOI: 10.1016/j.jneuroim.2014.09.014
Abstrakt: The role of microglia and monocyte-derived macrophages in experimental autoimmune encephalomyelitis pathogenesis has been controversial. To gain insight into their respective roles, we developed a method for differentiating between microglia and monocyte-derived macrophages in the CNS by flow cytometry utilizing anti-CD44 antibodies. We used this system to monitor changes in cell number, activation status, and gene expression by RNA sequencing over the course of disease. This in vivo characterization and RNA-Seq dataset improves our understanding of macrophage biology in the brain under inflammatory conditions and may lead to strategies to identify therapies for neuroinflammatory diseases.
(Copyright © 2014 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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