| Autor: |
Gambaryan PY; Biological Faculty, Moscow State University, Moscow, Russia., Kondrasheva IG; Research Center for Molecular Diagnostics and Therapy, Moscow, Russia., Severin ES; Research Center for Molecular Diagnostics and Therapy, Moscow, Russia., Guseva AA; Biological Faculty, Moscow State University, Moscow, Russia., Kamensky AA; Biological Faculty, Moscow State University, Moscow, Russia. |
| Jazyk: |
angličtina |
| Zdroj: |
Experimental neurobiology [Exp Neurobiol] 2014 Sep; Vol. 23 (3), pp. 246-52. Date of Electronic Publication: 2014 Sep 18. |
| DOI: |
10.5607/en.2014.23.3.246 |
| Abstrakt: |
To compare the efficacy of L-DOPA administered intranasally in the form of nanoparticles (nano-DOPA) and in standard drug forms using a rat Parkinson's Disease (PD) model. L-DOPA-containing nanoparticles (250±50 nm) were synthesized using the double emulsion method. The efficacy of nano-DOPA therapy was studied in Wistar rats with 6-OHDA-induced PD. Drugs were administered daily, 0.35 mg/kg (by L-DOPA). Animals' motor coordination and behavior were analyzed using the forelimb placing task and several other tests. Thirty minutes after the first administration, animals treated with L-DOPA, L-DOPA+benserazide, and nano-DOPA showed equally significant (p<0.05) improvements in coordination performance in comparison to the non-treated group. After 4 weeks of treatment, coordination performance in the nano-DOPA group (89±13% of the intact control level) was twice as high as in the L-DOPA and L-DOPA+benserazide groups, which did not differ from non-treated animals. The effect of nano-DOPA was significantly higher and more long-lasting (90±13% at 24 h after administration); moreover, it was still significant one week after the treatment was discontinued. Intranasal nano-DOPA was found to provide a lasting motor function recovery in the 6-OHDA-induced rat PD model with the effect sustained for one week after discontinuation, while the same doses of standard drugs provided significant effect only after the first administration. L-DOPA administered in the form of PLGA-based nanoparticles had a higher effective half-life, bioavailability, and efficacy; it was also efficiently delivered to the brain by intranasal administration. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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