Neurotoxic action of microcystin-LR is reflected in the transcriptional stress response of Caenorhabditis elegans.

Autor: Saul N; Department of Biology, Freshwater and Stress Ecology, Humboldt-Universität zu Berlin, Späthstr. 80/81, 12437 Berlin, Germany. Electronic address: nadines1976@aol.com., Chakrabarti S; Department of Biology, Freshwater and Stress Ecology, Humboldt-Universität zu Berlin, Späthstr. 80/81, 12437 Berlin, Germany., Stürzenbaum SR; School of Biomedical Sciences, Analytical and Environmental Sciences Division, King's College London, 150 Stamford Street, London SE1 9NH, UK., Menzel R; Department of Biology, Freshwater and Stress Ecology, Humboldt-Universität zu Berlin, Späthstr. 80/81, 12437 Berlin, Germany., Steinberg CE; Department of Biology, Freshwater and Stress Ecology, Humboldt-Universität zu Berlin, Späthstr. 80/81, 12437 Berlin, Germany.
Jazyk: angličtina
Zdroj: Chemico-biological interactions [Chem Biol Interact] 2014 Nov 05; Vol. 223, pp. 51-7. Date of Electronic Publication: 2014 Sep 23.
DOI: 10.1016/j.cbi.2014.09.007
Abstrakt: Cyanobacterial blooms in aquatic environments are frequently characterized by elevated levels of microcystins, a potent hepatotoxin. Here we exposed the nematode Caenorhabditis elegans with environmentally realistic concentrations of MC-LR to explore its non-hepatic toxicity. Lifespan, reproduction and growth assays confirmed the toxic potential of 100μg/L MC-LR even in this liver-lacking invertebrate. Whole-genome microarray analysis revealed that a neuromodulating action was the dominant response in nematodes challenged with 100μg/L MC-LR. Indeed, most of the 201 differentially expressed genes were associated with neurobehavior, neurogenesis, and signaling associated pathways. In addition, a whole-genome miRNA-microarray highlighted that, in particular, members of the let-7 family were differentially regulated. These miRNAs are involved in the developmental timing of cell fates, including neurons, and are probably also part of the stress response system. To conclude, neurological modulation is the main transcriptional stress response in C. elegans exposed to MC-LR.
(Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.)
Databáze: MEDLINE
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