Tumor-induced alterations in lymph node lymph drainage identified by contrast-enhanced MRI.

Autor: Ruddell A; Fred Hutchinson Cancer Research Center, Seattle, WA, USA.; Department of Comparative Medicine, University of Washington, Seattle, WA, USA., Kirschbaum SB; Fred Hutchinson Cancer Research Center, Seattle, WA, USA., Ganti SN; Department of Comparative Medicine, University of Washington, Seattle, WA, USA., Liu CL; Seattle Cancer Care Alliance, Seattle, WA, USA., Sun RR; Seattle Cancer Care Alliance, Seattle, WA, USA., Partridge SC; Seattle Cancer Care Alliance, Seattle, WA, USA.; Department of Radiology, University of Washington, Seattle, WA, USA.
Jazyk: angličtina
Zdroj: Journal of magnetic resonance imaging : JMRI [J Magn Reson Imaging] 2015 Jul; Vol. 42 (1), pp. 145-52. Date of Electronic Publication: 2014 Sep 25.
DOI: 10.1002/jmri.24754
Abstrakt: Background: To use high resolution MRI lymphography to characterize altered tumor-draining lymph node (TDLN) lymph drainage in response to growth of aggressive tumors.
Methods: Six mice bearing B16-F10 melanomas in one rear footpad were imaged by 3.0 Tesla (T) MRI before and after subcutaneous injection of Gadofosveset trisodium (Gd-FVT) contrast agent into both rear feet. Gd-FVT uptake into the left and right draining popliteal LNs was quantified and compared using Wilcoxon signed-rank test. Fluorescent dextran lymphography compared patterns of LN lymph drainage with the pattern of immunostained lymphatic sinuses by fluorescence microscopy.
Results: TDLNs exhibited greater Gd-FVT uptake than contralateral uninvolved LNs, although this difference did not reach significance (P < 0.06). Foci of contrast agent consistently surrounded the medulla and cortex of TDLNs, while Gd-FVT preferentially accumulated in the cortex of contralateral LNs at 5 and 15 min after injection. Fluorescent dextran lymphography confirmed these distinct contrast agent uptake patterns, which correlated with lymphatic sinus growth in TDLNs.
Conclusion: 3.0T MRI lymphography using Gd-FVT identified several distinctive alterations in the uptake of contrast agent into TDLNs, which could be useful to identify the correct TDLN, and to characterize TDLN lymphatic sinus growth that may predict metastatic potential.
(© 2014 Wiley Periodicals, Inc.)
Databáze: MEDLINE
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