| Autor: |
Koenderman AH; Department of Medical Chemistry, Vrije Universiteit, Amsterdam, The Netherlands., Koppen PL, Koeleman CA, van den Eijnden DH |
| Jazyk: |
angličtina |
| Zdroj: |
European journal of biochemistry [Eur J Biochem] 1989 May 15; Vol. 181 (3), pp. 651-5. |
| DOI: |
10.1111/j.1432-1033.1989.tb14772.x |
| Abstrakt: |
A specific and fast method for the determination of N-acetylglucosaminyltransferase III, IV and V activity in one assay is described. The method is based on the separation by HPLC of the three transferase products formed from the common acceptor oligosaccharide substrate GlcNAc beta 1----2Man alpha 1----3(GlcNAc beta 1----2Man alpha 1---- 6)Man beta 1----4GlcNAc. Assays are not interfered with by substances that result from enzymatic or chemical breakdown of the donor substrate UDP-[14C]GlcNAc. Using this assay system N-acetylglucosaminyltransferase III, IV and V activities were estimated in Novikoff ascites tumour cells, mouse lymphoma BW 5147 cells and hen oviduct. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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